摘要
目的:观察纳豆激酶对角叉菜胶所致的小鼠血栓模型的影响,为纳豆激酶相关产品的溶血栓功能性评价提供依据。方法:将雌性昆明种小鼠随机分为4组:空白组、模型对照组、低剂量组、高剂量组。连续灌胃30d,每天一次,于第30d,除空白组外,其他3组均用角叉菜胶制备尾部血栓模型,24h后观察并测量黑尾长度,之后摘眼球取血,测定血清纤维蛋白降解产物(FDP)、组织型纤溶酶原激活剂(t-PA)、组织型纤溶酶原激活剂抑制剂1(PAI-1)的含量。结果:各组小鼠最后一次称重体重无显著性差异;低剂量组与高剂量组的出栓率低于模型对照组,但模型对照组、低剂量组、高剂量组三组间相比较,黑尾长度、黑尾/全尾长的无显著性差异(p>0.05);高剂量组小鼠血清中的FDP及t-PA的含量显著高于模型对照组和低剂量组(p<0.05);空白组未检出PAI-1,其余各组PAI-1的含量无显著性差异。结论:纳豆激酶对实验小鼠体内血栓具有溶解作用,可以降低血栓的发生率;角叉菜胶诱导的小鼠血栓模型可以用于纳豆激酶溶(抗)血栓功能评价。
Objective.Observed effect of nattokinase on the tail-thrombus model,and provided basis for the function evaluation of nattokinase product. Methods.The mice were randomly allocated into four groups（each group with 12 mice）,including the normal group,the model control group,the low-dose nattokinase group,and the high-dose nattokinase group. Weighing up the mice was done every 7d. After the mice were fed for 30d, except for the normal group,other 3 groups were used to establish tail-thrombus models with carrageenan. After models were established for 24h,we observed the length of dark tails,and then detected the Fibrinogen degradation product（FDP）,tissue type plasminogen activator （t-PA）,and tissue type plasminogen activator inhibitor（PAI-1） in serum. Results.There was no significant difference between the length of dark tails among the model control group,the low-dose nattokinase group and the high-dose nattokinase group（p〉0.05）, but the dark tail rates of the later two group were lower than the former one. The values of FDP and t-PA of the high-dose nattokinase group were higher than those of the model control group and the low-dose nattokinase group（p〈0.05）, no PAl-1 was detected in the normal,and other groups had similar values of PAl-1. Conclusions： Nattokinase was effective in dissolving blood clots,and dropping incidence of disease. Carrageenan induced tail-thrombus model could be used to evaluate the antithrombotic and fibrinolysis enhancing effects of nattokinase.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第20期330-332,336,共4页
Science and Technology of Food Industry
基金
北京市教委科技计划面上项目(KM201211417005)
北京联合大学人才强校计划人才资助项目
