摘要
建立了高效液相色谱-示差折光方法来测定甜菊糖中莱鲍迪苷A含量的实验方法。色谱柱采用WatersNH2柱(250mm×4.6mm,5μm),以乙腈∶水=80∶20为流动相,采用示差折光检测器测定甜菊糖中莱鲍迪苷A的含量。莱鲍迪苷A的线性范围为0.1～1.0mg/mL,检出限为0.03mg/mL,平均加标回收率为98.37%,相对标准偏差0.88%。该方法简单、快速、准确、重现性好,适用于甜菊糖中莱鲍迪苷A的定量分析。
A high performance liquid chromatography method for separation and determination of rebaudioside A（RA） in stevia was established. The analytical column was Waters NH2（250mm×4.6mm,5um）,the mobile phase was acentonitrile-water（80：20,V/V）,the samples were determined by an refractive index detector detector. The calibration curves had good linearity in the range of 0.1-1.0mg/mL for RA,the detection limit for RA was 0.03mg/mL,the average recoveries of RA were 98.37% （n=6,RSD=0.88%）. The method was convenient, rapid and effective for qualitatively analysis of RA in stevia.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第20期64-65,69,共3页
Science and Technology of Food Industry
