摘要
为探讨血小板源生长因子(PDGF)在胃癌发生发展中的作用。对18例胃癌、36例慢性胃炎和15例对照组,应用快速尿素酶试验和Warthin—starry法检测HP;采用细胞生物活性法测定胃癌、胃炎组织和胃癌细胞株MGc803培养上清液中的PDGF活性。结果HP感染率在胃癌组和胃炎组无显著性差异。胃癌、胃炎组织和胃癌细胞培养上清液中均能测到PDGF活性,胃癌组要明显高于胃炎组。PDGF可明显促进胃癌细胞的体外增殖,且呈剂量依赖关系,抗—PDGF抗体却可明显抑制胃癌细胞的增殖,且呈剂量依赖关系,抗—PDGF抗体却可明显抑制胃癌细胞的增殖。结果提示PDGF的异常表达可能与胃癌的发病机理有关。
Helicobacter Pylori (HP) was examined in 18 patients with gastric carcinoma, 36 patients with chronic gastritis and controls by rapid urease test and Warthin - Starry method. Bioactivity assay was used to measure the production of PDGF by tissue of gastric carcinoma and gastritis and gastric carcinoma cell line MGC 803. The results showed that there was no significant difference in HP infection rate between gastric carcinoma and gastritis. PDGF activity was expressed in gastric carcinoma,gastritis and MGC803 cell line,and its expression level was higher in gastric carcinoma than in gastritis. PDGF obviously could promote MGC 803 cell proliferation and showing dose - dependent manner;but anti - PDGF antibody obviously could suppress MGC803 cell proliferation. PDGF may be associated with genesis of gastric carcinoma.
出处
《医学理论与实践》
2000年第6期323-325,共3页
The Journal of Medical Theory and Practice
关键词
胃癌
胃炎
血小板源生长因子
表达
Gastric carcinoma Gastritis Platelet - derived growth factor
