摘要
目的 探讨血小板源生长因子 (PDGF)在大肠癌的表达情况。方法 对 45例大肠癌患者和 10例正常大肠组织 ,应用免疫组化和原位杂交的方法 ,检测PDGF与PDGFmRNA的表达。并将结果与大肠癌的临床病理特征结合进行分析。结果 正常大肠组织没有PDGF和PDGFmRNA的表达 ,大肠癌的标本中 ,PDGD阳性率为 6 4.4% ,PDGFmRNA阳性率为 5 3.3 %。在浆膜浸润阳性、淋巴转移阳性、DukesC、D期患者中 ,PDGE及PDGFmRNA的表达显著增高 (P <0 .0 5 )。在有器官转移的患者 ,尽管 0 .0 5 <P <0 .10 ,但亦有表达的提高。而与年龄、性别、分化程度无关。结论 血小板源生长因子在大肠癌的演进过程中起到重要的作用。
Objective To study the expression of PDGF and PDGF?mRNA in human colorectal carcinoma.Methods Specimens of 45 colorectal carcinomas and 10 normal intestinal tissues were examined for PDGF expression by immunohistochemical staining and in situ hybridization, and the results were analyzed with the clinic materials.Results None of the normal intestinal tissues was PDGF or PDGF mRNA possitive.In the colorectal carcinoma patients,the positive rate of PDGF protein was 64.4% and the positive rate of PDGF mRNA was 53.3%.The expression of PDGF was significantly higher in the lymph nodes metastasis positive(P<0.05),the organ metastasis(0.05<P<0 10),serosa infiltrated (P<0.05) and the Dukes C、D patients(P<0.05).Positive PDGF staining was independent of age,sex and the degree of cell differentiation.Conclusion PDGF plays an important role in the progression of colorectal carcinoma.
出处
《第一军医大学分校学报》
2001年第1期7-9,共3页
Journal of Branch Campus of the First Military Medical University
