摘要
目的通过对过敏性紫癜惠儿T细胞亚群、血清免疫球蛋白、血清补体变化的分析，以进一步探讨儿童过敏性紫癜与免疫功能的关系，为临床提供实验依据。方法2011年1月～10月在西安交通大学医学院第一附属医院住院的过敏性紫癜患儿，过敏性紫癜的诊断均符合1990年美国风湿病协会（ARA）制定的过敏性紫癜诊断标准，共计45例，男26例，女19例，年龄2～14岁，平均年龄8．1岁。对照组：健康儿童共计20例，男10例，女10例，年龄3～13岁，平均年龄7．6岁。过敏性紫癜组性别、年龄与对照组相比，差异无统计学意义。应用多色流式细胞术检测T细胞亚群，应用免疫散射比浊法检测血清免疫球蛋白、血清补体。结果过敏性紫癜患儿T细胞亚群改变明显，CD3＋CD4＋（Th）显著低于对照组，t值为2．91，P〈O．01；CD3＋CD4＋（Th）／CD3＋CD8＋（Ts）比值降低，与对照组相比，t值为2．80，P〈0．01；过敏性紫癜患儿血清中IgA水平显著高于对照组，t值为2．76，P〈0．01；过敏性紫癜患儿血清补体指标与对照组相比，无差异。结论过敏性紫癜患儿存在细胞免疫和体液免疫功能异常，对过敏性紫癜患儿检测免疫功能有助于判断病情，指导治疗。
Objective To explore the relationship of children who had allergic purpura and immune function,also,provide the experimental basis for clinicalt hrough the analysis of allergic purpura in children with T-cell subsets,serum immunoglobu- lin＇s,and the change of serum complement. Methods Henoch-Schonlein （allergic） purpura in children hospitalized in the First Affiliated Hospital of Xi＇an Jiaotong University Medicine School from January to October in 2011, the diagnosis of allergic purpura were under the standards of allergic purpura diagnostic criteria which developed by the American Rheumatism Association （ARA） in 1990. There were total 45 cases,26 males and 19 females, from 2 to 14 years old;the average age was 8. 1 years. Control group：healthy children, there were total 20 cases, 10 males and 10 females, from 3 to 13 years old; the average age was 7.6 years. Compared the age and sex of allergic purpura group and the control group, the difference was not statistically significant. Used polychromatic flow cytometry to measure T cell subsets, and used turbidimetry immunohisto- chemical scattering to measure serum immunoglobulins and serum complement. Results There was obvious change in He- noch-Schonlein purpura in children with T cell subsets,CD3＋CIM＋ （Th） was significantly lower than those of the control group, t=2. 91, P〈0 01 ;of CD3＋CD4＋ （Th）/CD3 ＋CD8 ＋ （Ts）ratio was decreased, compared to the control group, t= 2.80, P〈0 01. The IgA levels of allergic purpura serum were significantly higher than those in the control group, t= 2.76, P〈0 01. Compared the serum complement indicators between the allergic children and the control group, there were no difference. Conclusion Henoch-Schonlein （allergic） purpura children have abnormal cellular and humoral immune function. So to detect the immune function of children with allergic purpura can help determine the condition and guide treatment.
出处
《现代检验医学杂志》
CAS
2012年第4期50-52,共3页
Journal of Modern Laboratory Medicine
关键词
过敏性紫癜
T细胞亚群
免疫球蛋白
血清补体
henoch-schonlein （allergic） purpura
T cell subsets
immunoglobulin
serum complement
