摘要
目的探索内蒙地区蒙古族儿童过敏性紫癜(AP)及其累及肾脏、胃肠道和关节与HLA-DRB1等位基因的关联性。方法引入PCR-SSP技术,在内蒙地区同是蒙古族但无血缘关系或蒙古族与其他民族间无通婚史及其他风湿性疾病史和家族史的蒙古族人群中,收集AP病例57例,其中累及肾脏20例、胃肠道29例和关节26例,分别与102名健康儿童的HLA-DRB1等位基因进行型别分析。组间比较在单因素χ2检验的基础上,作以多因素Logistic回归分析。结果HLA-DRB1*110x等位基因在AP及其累及关节、*070x等位基因在累及胃肠道分别与对照组经Logistic回归分析,均P<0.05,差异有显著意义。B均为正值,OR分别是2.251、2.215和2.552,均>1,与B值相符,95%可信区间内均不包含1。表明排除其他进入模型的等位基因作用外,DRB1*110等位基因在AP及其累及关节、*070x等位基因累及胃肠道的致病优势分别是对照组的2.251、2.215和2.552倍,促进发病。EF均>0;而DRB1*040x和*120x等位基因在AP病例组分别与对照组经Logistic回归分析,均P<0.05,差异有显著意义。B均为负值,OR分别是0.422和0.424,均<1,与B值相符,95%可信区间内均不包含1。表明排除其他进入模型等位基因作用外,DRB1*040x和*120x等位基因在AP病例组致病优势分别是对照组的0.422和0.424倍,阻止发病。PF均>0。结论HLA-DRB1*110x等位基因可能为内蒙地区蒙古族儿童AP及其累及关节、*070x等位基因为累及胃肠道发病单体型中的遗传易感基因,而DRB1*040x和*120x等位基因为AP的遗传保护基因。
Objective To explore the correlation between HLA-DRB1 allele and anaphylactoid purpura（AP） and its association with kidney,gastrointestinal and joint in juvenile of Mongolian residing in Inner Mongolia. Methods We collected 57 Mongolian children with AP,who had been residing in lnner Mongolia three generations,had not kinship,intermarriage background,other medical history and family history of rheumatic with each other,20 of them involing kidney, 29 involving gastrointestinal, 26 involving joint and compared rheir polymorphism of HLA- DRB1 allele with 102 health children＇ s respectively, and leaded into PCR-SSP technigne. The comparing between group and group was dealt with by logistic regression after one-way X^2 test. Results The logistic regression between HLA-DRB1 110x allele in AP and its association with joint, 070x allele in involving gastrointestinal and control group respectively were dealt with. P 〈 0.05 for all, the deffirence had statistical significance. B were positive for all, OR were 2.251,2.215 and 2. 552 respectively, all〉 1, whose 95 % confident interval didn＇ t include 1 for all. It indicated that the led to disease odds of DILB1 ＊ 110x allele in AP and its association with joint, ＊ 070x allele in involving gastrointestinal were 2. 251,2. 215 and 2. 552 times of control group respectively except for action of other alleles of get into pattern,and it was led to pathogenesis. EF were 〉 0 for all, While the Logistic regression between DRB1＊040x and＊ 120x alleles in AP and control group respectively were dealt with. P were 〈 0.05 for all, the deffirence had statistical significance. B were negative for all, OR were 0. 422 and 0. 424 respectively, all 〈 1, whose 95 % confident intervol didn＇t include 1 for all. It＇s indicated that the led to disease odds of DRB1＊040x and 120x allele in AP were 0. 422 and 0. 424 times of control group respectively except action of other alleles of get into pattern,and It was protective pathogenesis. PF were 〉 0 for all. Conclusion The allele of HLA-DRB1 ＊ 110x may be a susceptible gent in AP and its associated with joint and ＊＊ 070x is a susceptible gene in AP associated with gastoointestinal;While the alleles of DRB1 ＊ 040x and ＊ 120x are the protective ones in AP of children who are Mongolian inhabitations in Inner Mongolia.
出处
《中国基层医药》
CAS
2005年第12期1659-1661,共3页
Chinese Journal of Primary Medicine and Pharmacy
基金
内蒙古科委自然科学基金(990302-1)
