摘要
目的观察骨髓间充质干细胞(BMSCs)移植治疗多器官功能不全综合征(MODS)模型兔的临床及心、肺、肝、肾等器官的病理改变。方法将35只兔子随机分为3组:正常对照组5只,模型组15只,模型移植治疗组15只。BMSCs体外培养、扩增、鉴定后,通过股动脉移植到患有MODS模型兔左心室。第28天处死各组兔后取器官进行病理切片。结果 BMSCs培养能够贴壁成长,具有分化能力。模型组兔精神萎靡,反应迟钝,结膜苍白,口唇发绀,部分动物出现呼吸急促,双肺湿罗音,心、肺、肝、肾等器官出现淤血、水肿、变性坏死,炎细胞浸润等改变;模型移植治疗组临床症状明显减轻,病理改变明显减轻接近对照组。结论 BMSCs具有良好的生物学活性,移植后通过整合能替代凋亡坏死细胞并对MODS起到治疗作用,对MODS组织修复有一定疗效。
Objective To observe clinical symptoms and pathological changes in the heart,lung,liver and kidney of rabbits with multiple organ dysfunction syndrome（MODS） following bone marrow mesenchymal stem cells（BMSCs） transplantation. Methods 35 rabbits were randomly divided into three groups：the normal control group（n = 5）, the model group（n = 15） and the treatment group（n~ 15）. Rabbit BMSCs were cultured,amplified and identified in vitro,and then, transplanted left ventricle of MODS model rabbits through femoral artery. Each group rabbits were executed on the day of 28th and the organs were harvested for pathological biopsy. Results BMSCs could stick wall growth,with differentiation capacity. The model group rabbits were listlessness,unresponsive, pale conj unctiva, cyanotic lips, part of them polypnoea, moist rale in double lung, congestion, dropsy, degeneration necrosis and inflammation etc. changes in heart, lungs, liver, kidney and so on. The treatment group rabbits＇ clinical symptoms obviously reduced, pathological change significantly reduced close to the control group. Conclusion Bone marrow mesenchymal stem cells showed good bioactivity, through integration could substituted apoptosis and necrosis cells, had therapeutic effects on multiple organ dysfunction syndrome,with certain curative effect in tissue repair for MODS.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第29期3074-3075,3078,I0001,I0002,共5页
Chongqing medicine
关键词
骨髓间充质干细胞
多器官功能衰竭
移植
病理改变
bone marrow mesenchymal stern cells, multiple organ failure
transplant
pathological change
