摘要
目的建立一种接近临床的失血性休克合并内毒素血症致多器官功能障碍综合征(MODS)动物模型。方法预实验组取32只家兔,股动脉放血诱发休克,随后回输全部血液。血压平稳后,在腹腔注射不同计量的内毒素(LPS,各8只),观察各组动物相应时间点(12、24、48h)的一般情况和死亡率。实验组分为模型组和正常对照组(各8只)。根据预实验结果,选定休克并10μg/kgLPS损伤动物为MODS模型组,进行上述相应时间点血浆炎性递质各个器官功能检测,并行病理学检查。结果股动脉放血快速回输并注射10μg/kgLPS组家兔MODS的死亡率在12、24和48h分别为12.5%、50.0%和62.5%,较符合MODS的临床表现。模型组动物在内毒素注入后12h已出现全身炎症反应综合征,24～48h各器官功能指标均出现2倍以上不同程度的升高,并伴有血浆肿瘤坏死因子α(TNFα)、白细胞介素6(IL6)的升高,明显的低血氧症和高碳酸血症。病理学观察显示肺、肝、小肠等出现不同程度的实质性损伤。结论该实验较好的重现了失血性休克合并内毒素血症致MODS的诱因和临床特点,为临床实验研究提供了一种可行性的MODS动物模型。
Objective To establish a standard rabbit model that would imitate the clinical features of multiple organ dysfunction syndrome(MODS) induced by hemorrhagic shock combined with endotoxin.Methods A rabbit model of hemorrhagic shock was established by bleeding and maintaining blood pressure within 40mmHg for 1h,followed by rapid resuscitation with Ringer's lactate and shed blood.Then rabbits were divided into 4 groups in random and subjected to different dose of endotoxin(LPS) to determine the dose to cause MODS.The parameters of important organ functions were measured,changes of pathology were observed by microscopy,and TNF-α,IL-6 were studied by ELISA.Results The animals in 10μg/kg LPS injection group all presented the characteristic systemic imflammatory response syndrone (SIRS) and the mortality rate was parallel to the clinical situation.Occurrence of SIRS,significant increase of organ function parameters and changes of pathology further confirmed that MODS was induced through this method.Conclusion This model replicated well the clinical characteristics of MODS associated with hemorrhagic shock followed by endotoxin.
出处
《江苏医药》
CAS
CSCD
北大核心
2005年第6期448-450,共3页
Jiangsu Medical Journal
