期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

Grim-19对宫颈癌HeLa细胞端粒酶活性及衰老的影响 被引量：3

The effect of GRIM-19 gene on the telomerase activation and cell senescence in the cervical cancer cell line HeLa
暂未订购
导出
摘要 目的重组质粒转染HeLa细胞以探讨Grim-19表达水平的变化对HeLa细胞中端粒酶活性的影响。方法将重组质粒p3×flag-myc-cmv-24-Grim-19(简写Grim-19)通过脂质体转染至HeLa细胞,48 h后通过Western blot检测Grim-19、htert、P16、P21的表达情况,同时检测HeLa细胞衰老和端粒酶活性。结果转染后可见flag的表达及htert的表达降低及P16、P21的表达升高,细胞衰老增加,增殖减弱及端粒酶活性的降低。结论在子宫颈癌细胞株HeLa中Grim-19具有调控端粒酶活性和细胞衰老的作用。 Objective To study the effect of the changing expression of Grim-19 on the telomerase activity in HeLa cell which was transfected from recombinant plasmid. Methods After the plasmid p3 × flag-myc-cmv-24-Grim-19 （Grim-19） was transfeeted into the HeLa cells, Western blot were used to test the expression levels of Grim-19, htert, P16 and P21 after 48 h. The senescence and telomerase activity of HeLa cell were also detected. Results The low expression of flag and htert and overexpression of P16 and P21 can be obvious after the transfection, and the increasing cellular senescence and weakening proliferation and reducing telomerase activity were also found to be- true. Conclusion Grim-19 can regulate the telomerase activity and cell senescense in the cervical cancer cell line.
作者 杨霞 王青元 朱靖 冯定庆 朱园园 周颖 凌斌
机构地区 安徽医科大学附属省立医院妇产科
出处 《安徽医科大学学报》 CAS 北大核心 2012年第10期1152-1155,共4页 Acta Universitatis Medicinalis Anhui
基金 国家自然科学基金(编号:81008168 81072127) 安徽省高等学校省级自然科学研究项目(编号:KJ2010B375)
关键词 GRIM-19 子宫颈腺癌 HELA细胞 端粒酶 转染 Grim-19 cervical adenocarcinoma HeLa cell telomerase transfection
分类号 R737.33 [医药卫生—肿瘤]
  • 相关文献

参考文献6

  • 1Kalvakolanu D V. The GRIMs: a new interface between cell death regulation and interferon/retinoid induced growth suppression [ J ]. Cytokine Growth Factor Rev ,2004, 15 ( 2-3 ) : 169 - 94.
  • 2周颖,凌斌.GRIM-19的功能及与肿瘤的相关性[J].医学分子生物学杂志,2008,5(3):262-264. 被引量：30
  • 3Angell J E, Lindner D J, Shapiro P S, et al. Identification of GRIM-19, a novel cell death-regulatory gene induced by the inter- feron-beta and retinoic acid combination, using a genetic approach [J]. J Biol Chem,2000,275(43):33416-26.
  • 4李敏(综述),周颖(审阅),凌斌(审阅).端粒及端粒酶活性的调控机制[J].医学分子生物学杂志,2010,7(2):176-179. 被引量：6
  • 5Haley D M, West S C, Beattie T L. InTERTpreting telomerase structure and function[J]. Nucleic Acids Res, 2010, 38 (17) : 5609 - 22.
  • 6Gourronc F A, Robertson M, Herrig A K, et al. Proliferative de- fects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase TERT, or by activation of endogenous telomerase through expression of papillo- mavirus E6/E7 or the telomerase RNA component, TERC [ J ]. Exp Dermatol, 2010,19 ( 3 ) :279 - 88.

二级参考文献21

  • 1龚龙波,罗学来,刘双又,陶德定,龚建平,胡俊波.GRIM-19及其靶基因产物STAT3与结直肠癌恶性程度的关系[J].癌症,2007,26(7):683-687. 被引量：43
  • 2TICHON A,GOWDA B K,SLAVIN S,et al.Telomerase activity and expression in adult human mesenchymal stem cells derived from amyotrophic lateral sclerosis individuals[J].Cytotherapy,2009,11(7):837-848.
  • 3BAZAROV A V,HINES W C,MUKHOPADHYAY R,et al.Tel-omerase activation by c-Myc in human mammary epithelial cells requires additional genomic changes[J].Cell Cycle,2009,8(20):3373-3378.
  • 4LIU L,ISHIHARA K,ICHIMURA T,et al.MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity[J].J Biol Chem,2009,284(8):5165-5174.
  • 5LIU X,DAKIC A,ZHANG Y,et al.HPV E6 protein interacts physically and functionally with the cellular telomerase complex[J].Proc Natl Acad Sci USA,2009,106(44):18780-18785.
  • 6CALADO R T,YEWDELL W T,WILKERSON K L,et al.Sex hormones,acting on the TERT gene,increase telomerase activity in human primary hematopoietic cells[J].Blood,2009,114(11):2236-2243.
  • 7HAN Y,SAN-MARINA S,YANG L,et al.The zinc finger domain of Wilms'tumor 1 suppressor gene(WT1)behaves as a dominant negative,leading to abrogation of WT1 oncogenic potential in breast cancer cells[J].Breast Cancer Res,2007,9(4):R43.
  • 8CHOU C K,LEE D F,SUN H L,et al.The suppression of MAD1 by AKT-mediated phosphorylation activates MAD1 target genes transcription[J].Mol Carcinog,2009,48(11):1048-1058.
  • 9LIU X,ROBERTS J,DAKIC A,et al.HPV E7 contributes to the telomerase activity of immortalized and tumorigenic cells and augments E6-induced hTERT promoter function[J].Virology,2008,375(2):611-623.
  • 10MORRISON A A,VINEY R L,LADOMERY M R.The posttransc-riptional roles of WT1,a multifunctional zinc-finger protein[J].Biochim Biophys Acta,2008,1785(1):55-62.

共引文献34

同被引文献36

  • 1Parkin DM,Bray F,Ferlay J,et al.Global cancer statistics,2002[J].CA Cancer J Clin,2005,55(2):74-108.
  • 2Gourronc FA,Robertsonm M,Herrig AK,et al.Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase,TERT,or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA component,TERC[J].Exp Dermatol,2010,19(3):279-288.
  • 3Singh A,Sharma H,Salhan S,et al.Evaluation of expression of apoptosis-related proteins and their correlation with HPV,telomerase activity,and apoptotic index in cervical cancer[J].Pathobiology,2004,71(6):314-322.
  • 4Ferber MJ,Montoya DP,Yu C,et al.Integrations of the Hepatitis B virus(HBV)and human papillomavirus(HPV)into the human telomerase reverse transcriptase(h TERT)gene in liver and cervical cancers[J].Oncogene,2003,22(24):3813-3820.
  • 5Jeong Seo E,Jung Kim H,Jae Lee C,et al.The role of HPV oncoproteins and cellular factors in maintenance of h TERT expression in cervical carcinoma cells[J].Gynecol Oncol,2004,94(1):40-47.
  • 6Hopman AH,Theelen W,Hommelberg PP,et al.Genomic integration of oncogenic HPV and gain of the human telomerase gene TERC at 3q26 are strongly associated events in the progression of uterine cervical dysplasia to invasive cancer[J].J Pathol,2006,210(4):412-419.
  • 7Heselmeyer-Haddad K,Sommerfeld K,White NM,et al.Genomic amplification of the human telomerase gene(TERC)in pap smears predicts the development of cervical cancer[J].Am J Pathol,2005,166(4):1229-1238.
  • 8Caraway NP,Khanna A,Dawlett M,et al.Gain of the 3q26 region in cervicovaginal liquid-based pap preparations is associated with squamous intraepithelial lesions and squamous cell carcinoma[J].Gynecol Oncol,2008,110(1):37-42.
  • 9Kurvinen K,Syrjnen S,Johansson B.Long-term suppression of telomerase expression in He La cell clones,transfected with an expression vector carrying siRNA targeting h TERT mRNA[J].Int J Oncol,2006,29(1):279-288.
  • 10Alameda F,Espinet B,Corzo C,et al.3q26(h TERC)gain studied by fluorescence in situ hybridization as a persistence-progression indicator in low-grade squamous intraepithelial lesion cases[J].Hum Pathol,2009,40(10):1474-1478.

引证文献3

二级引证文献1

安徽医科大学学报
2012年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部