摘要
目的:应用基因表达谱芯片技术筛查上调微小RNA-205(miR-205)表达后人宫颈腺癌细胞株HeLa细胞中差异表达基因,并探讨其功能及上调miR-205致癌机制。方法:应用含有23 232条人类全长基因的cDNA表达谱芯片,对上调miR-205表达的HeLa细胞及HeLa细胞原株的基因表达谱进行分析。结果:与HeLa细胞原株相比,上调miR-205表达的HeLa细胞中有差异表达的基因共172条,其中34条上调,138条下调。结论:基因芯片技术能够有效筛查出上调miR-205表达后HeLa细胞中差异表达基因,miR-205引发宫颈癌是多因素多基因共同作用的结果。
Objective：To identify a set of genes related to upregulated expression of miR-205 in HeLa cells and to investigate their function,and to discuss the pathogenesis of cervical cancer.Methods：The HeLa cells upregulated expression of miR-205 and normal HeLa cells were analyzed with 23 232 human genes of cDNA microarrays.Results：A total of 172 differential genes expressed in the HeLa cells upregulated expression of miR-205 were screened out,of which,34 were up regulated and 138 were down regulated.Conclusions：The cDNA microarray is a powerful method to identify the gene expression profile.Cervical cancer induced by upregulated expression of miR-205 is a result of coeffecting by polygene and multiple factors.
出处
《国际妇产科学杂志》
CAS
2012年第5期518-520,共3页
Journal of International Obstetrics and Gynecology
基金
国家自然科学基金(81101379)
广东省自然科学基金(S2011040004639)
广东省医学科研基金(B2011088)
关键词
宫颈肿瘤
HELA细胞
芯片分析技术
微RNAS
基因表达
Uterine cervical neoplasms
Hela cells
Microchip analytical procedures
MicroRNAs
Gene expression
