摘要
目的:探讨表没食子儿茶素没食子酸酯(EGCG)对人宫颈癌细胞株HeLa的作用机制。方法:将对数生长期的HeLa细胞用不同剂量的EGCG(0,10,20,40μmol)处理。细胞培养72h后,MTT法检测细胞增殖,流式细胞术检测细胞凋亡,Western blot法检测细胞磷酸化-丝裂原活化蛋白激酶(p-p38)、丝裂原活化蛋白激酶(p38)、磷酸化核因子-κB(p-NF-κB)、核因子-κB(NF-κB)、B细胞淋巴因子2(Bcl-2)、Bcl-2相关X蛋白(Bax)、活化半胱胺酸蛋白酶蛋白3(Cleaved-Caspase-3)表达。ELISA和RT-PCR法分别检测细胞上清和细胞内肿瘤坏死因子α(TNF-α)、白介素-1β(IL-1β)和白介素-8(IL-8)表达。结果:EGCG可呈剂量依赖性诱导HeLa细胞增殖抑制与凋亡,并可增加Bax和cleavedCaspase3表达,下调p-p38、p-NF-κB、Bcl-2、TNF-α、IL-1β和IL-8表达,而对p38和NF-κB表达无明显影响。结论:EGCG可诱导HeLa细胞增殖抑制和凋亡,其作用机制与抑制p38/NF-κB信号通路介导的炎症相关。
Objective：To investigate the effect and mechanisms of EGCG on the human cervical cancer cells lines HeLa.Methods：The HeLa cell at Logarithmic growth phase were treated with EGCG（ 0,10,20,40μmol） for 72 h.MTT and flow cytometric analysis were used to evaluate the proliferation and the apoptosis of HeLa cell,respectively.The expressions of phosphorylation p38 mitogen-activated protein kinase（ p-p38）,p38,phosphorylation nuclear transcription factorsp-κB（ NF-κB）,NF-κB,Bax,Bcl-2 and cleaved-Caspase3 in cell were detected by Western blot.The expressions of TNF-α,IL-1β and IL-8 in cell and Cell supernatant were examined by RT-PCR and ELISA,respectively.Results：EGCG can significantly decrease the proliferation of HeLa and reduce the expressions of p-p38,p-NF-κB,Bcl-2,TNF-α,IL-1β and IL-8 in a dose-dependent manner.Further,EGCG treatment also can increase the apoptosis of HeLa and enhance the expression of cleaved-caspase3 and Bax in a dose-dependent manner with no influence on the expression of p38 and NF-κB.Conclusion：EGCG can induces the apoptosis and growth inhibition of HeLa.The mechanism of which is involved in suppressing p38 /NF-κB mediating inflammation.
出处
《现代妇产科进展》
CSCD
北大核心
2017年第1期37-39,43,共4页
Progress in Obstetrics and Gynecology
