摘要
【目的】为了初步建立野生‘毛桃1号’叶片愈伤组织诱导、保存及不定芽再生技术体系,【方法】以野生‘毛桃1号’试管苗幼嫩叶片为外植体,探讨了基本培养基、激素配比等一系列因素对‘毛桃1号’叶片愈伤组织诱导和保存的影响及TDZ浓度对愈伤组织不定芽再生的影响。【结果】结果表明,适合‘毛桃1号’叶片愈伤组织诱导的培养基为LP+6-BA 1.0 mg.L-1+2,4-D 3.0 mg.L-1+AgNO30.5 mg.L-1+蔗糖30.0 g.L-1+琼脂6.0 g.L-1;保存的培养基为LP+2,4-D1.5 mg.L-1+CH 0.4 g.L-1+蔗糖30 g.L-1+CA 3.0 g.L-1或PVP 3.0 g.L-1+琼脂5.8 g.L-1,且采用持续暗培养保存,每28 d继代1次。愈伤组织在SH+TDZ 3.0 mg.L-1+NAA 0.3 mg.L-1+蔗糖30 g.L-1+琼脂6.0 g.L-1的培养基上可再生不定芽,再生率为6.83%。【结论】初步建立了‘毛桃1号’叶片愈伤组织诱导、保存及不定芽再生体系,为桃愈伤组织再生和遗传转化的研究奠定了基础。
[Objective] The objective of the study is to establish the system of callus induction and adventitious shoot regeneration from leaves of ‘Maotao No. 1.＇ [Method]Experiment was conducted with the young leaves in vitro for studying the effects of different basic medium, plant growth regulator types and content and other factors on the callus induction and conservation. [Result] The results showed that the optimal medium for callus induction from leaves was LP containing 1.0 mg·L^（-1） 6-BA, 3.0 mg· L^（-1） 2, 4-D, 0.5 mg·L^（-1） AgNO3, 30.0 g. L^（-1） sucrose and 6.0 g·L^（-1） agar, while for callus conservation was LP containing 1.5 mg·L^（-1） 2,4-D, 0.4 g·L^（-1）CH, 3.0 g·L^（-1） CA or 3.0 g·L^（-1）PVP, 30.0 g·L^（-1） sucrose and 5.8 g·L^（-1） agar. The calli were maintained in the dark environment and transferred to fresh medium every 28 d. The suitable medium for the adventitious shoot regeneration from the calli was SH supplemented with 0.3 mg·L^（-1） NAA, 3.0 mg·L^（-1） TDZ, 30.0 g· L^（-1） sucrose and 6.0 g·L^（-1） agar, with 6.83% of regeneration rate.
出处
《果树学报》
CAS
CSCD
北大核心
2012年第5期814-819,F0004,共7页
Journal of Fruit Science
基金
国家桃产业技术体系建设专项(CARS-31-2-4)
关键词
'毛桃1号’
愈伤组织
植株再生
The studies established the system of callus induction and adventitious shoot regenerationfrom leaves of ‘ Maotao No. 1＇, which lays foundation for the peach callus regeneration and genetictransformation.
