摘要
目的 探讨双歧杆菌表面分子脂磷壁酸 (LTA)对体外培养的HL 6 0白血病细胞端粒酶活性的影响。方法 采用PCR ELISA法检测经LTA处理前后的HL 6 0白血病细胞株端粒酶活性的改变。结果 经LTA处理后 ,HL 6 0白血病细胞的生长受到抑制 ,端粒酶活性明显降低。结论 双歧杆菌LTA对HL 6 0白血病细胞具有生长抑制作用 ,其抗肿瘤作用的机理可能与抑制肿瘤细胞的端粒酶活性有关。
Objective To investigate effects to telomerase activity by lipoteichoic acids (LTA) of Bifidobacterium in HL-60 cells in culture. Methods MTT assay was used to investigate the inhibitory effect of LTA on HL-60 cell in culture. The telomerase activity in cells treated by LTA was detected by PCR-ELISA assay which is based upon PCR amplification of the initial telomerase product and detected by ELISA. Results The growth of HL-60 cells was inhibited by 20μg/ml LTA and the inhibitory effect was time-and dose-dependent. After being treated with 40μg/ml LTA for 3 days, the telomerase activity in HL-60 cells was significantly reduced to about 34.2% of that in untreated control. Conclusion The lipoteichoic acids of Bifidobacterium could directly inhibit the growth of HL-60 cells. The mechanism of anti-tumor by lipoteichoic acid may be explained by the inhibition of telomerase activity in cells by LTA.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2000年第4期316-318,共3页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金!资助项目 (39870 0 0 7)
