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双歧杆菌脂磷壁酸对NF-κB在结肠癌细胞中表达的影响 被引量：2

Effect of lipoteichoic acid of Bifidobacterium on NF-κB expression in colon cancer cells
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摘要 目的探讨双歧杆菌脂磷壁酸(LTA)对NF-κB在结肠癌细胞HCT-116中表达的影响。方法采用免疫细胞化学染色、PAGE及Western blot等,分析经双歧杆菌LTA处理前后,NFκ-B蛋白在结肠癌细胞HCT-116中表达的差异。结果经LTA处理后,NFκ-B在HCT-116细胞中的表达明显降低,且呈剂量依赖性。结论双歧杆菌LTA可下调NF-κB的表达,对了解LTA诱导HCT-116细胞凋亡的机制提供了一定的实验依据。 Objective To explore the effect of lipoteichoic acid （LTA） of Bifidobacterium on NF-κB expression in colon cancer cells HCT-116. Method Differences of NF-κB expression of HCT-116 cells were detected by Immunocytochemical staining, PAGE and Western blot, before and after treatment with different dose of LTA of Bifidobacterium. Result After treatment with different dose of LTA, the NF-κB expression in HCT-116 cells notably decreased in dose-dependent manner. Conclusion LTA of Bifidobacterium can downregulate NF-κB expression in HCT-116 cells, which provides some bases for understanding the mechanism of HCT-116 cell apoptosis induced by LTA of bifidobacterium.
作者 王跃 罗强 刘明方 陈淑惠
机构地区 重庆医科大学临床微生物学教研室
出处 《中国微生态学杂志》 CAS CSCD 2006年第5期348-349,352,共3页 Chinese Journal of Microecology
基金 重庆市教委科研项目(040308)
关键词 双歧杆菌 脂磷壁酸 结肠癌 NF-ΚB 表达 Bifidobacterium Lipoteichoic acid Colon cancer NF-κB Expression
分类号 R735.35 [医药卫生—肿瘤]
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参考文献10

  • 1VOLKMAN B F,DEBAHOV D V,ZHANG Q,et al.Biosynthesis of D-alanyl lipoteichoic acid:the tertiary structure of apoD-alanylcarrier protein[J].Biochemistry,2001,40(27):7964-7972.
  • 2OKAMOTO M,OSHIKAWA T,OHE G,et al.Severe impairment of anti-cancer effect of lipoteichoic acid-related molecule isolated from a penicillin-killed streptococcus pyogenes in toll-like receptor 4-deficient mice[J].Int Immunopharmacol,2001,1 (9-10):1789-1795.
  • 3SUZUKI N,SUZUKI S,DUNCAN D,et al.Severe impairment of IL-1 and TLR signaling in mice lacking IRAK-4[J].Nature,2002,416(6882):750-756.
  • 4MEDZHITOV R,PRESTON H P,JANEWAY C A Jr.A human homologue of the Drosophila Toll protein signals activation of adaptive immunity[J].Nature,1997,388(6640):394-397.
  • 5JANSSENS S,BURNS K,TSCHOPP J,et al.Regulation of IL-1 and LPS induced NF-kappaB activation by alternative splicing of MyD88[J].Curr Biol,2002,12(6):467-471.
  • 6HULL C,MCLEAN G,WONG F,etal.Lipopolysaccharide signals an endothelial apoptosis pathway through TNF receptor-associated factor 6-mediated activation of c-Jun NH2-terminal kinase[J].J Immunol,2002,169(5):2611-2618.
  • 7宋淑霞,冯旭,蔡月花,吕占军.益气补肾药方对老龄小鼠T细胞中NF-κB活性的影响及作用机制[J].细胞与分子免疫学杂志,2004,20(2):239-241. 被引量：1
  • 8HUERTA-YEPEZ S,VEGA M,JAZIREHI A,et al.Nitric oxide sensitizes prostate carcinoma cell lines to TRAIL-mediated apoptosis via inactivation of NF-kappaB and inhibition of Bcl-(xL)expression[J].Oncogene,2004,23(29):4993-5003.
  • 9]LOTZ S,AGA E,WILDE I,et al.Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2[J].J Leukoc Biol,2004,75(3):467-477.
  • 10GEWIRTZ A T.Intestinal epithelial toll-like receptors:to protect and serve?[J].Curr Pharm Des,2003,9(1):1-5.

二级参考文献8

  • 1Effros RB. Ageing and the immune system[J]. Novartis Found Symp,2001 , 235:130 -149.
  • 2Negoro S, Hara H, Myata S, et al. Mechanisms of age-related declinein antigen-specific T cell proliferative response: IL-2 receptor expression and recombinant IL-2 induced proliferative response of purifiedTac-positive T cell[J]. Mech Aging Dev, 1986, 36:
  • 3Shibuya H, Yoneyama M, Taniguchi T. Involvement of a commontranscription factor in the regulated expression of IL-2 and IL-2R receptor genes[J]. Int Immunol, 1989, 1: 43 -49.
  • 4Trebilcock GU, Ponnappan U. Induction and regulation of NF-κB during aging: role of protein kinases[ J]. Clin Immunol Immunopathol,1996, 79(1): 87 -91.
  • 5Ponnappan U. Regulation of transcription factor NFkappa B in immunesenescence[ J ]. Front Biosci, 1998, 1 (3): 152 - 168.
  • 6Jorge C, Cristina T, Guifang C, et al. Identification of a role for NFκB in the regulation of apoptosis and in maintenance of T cell-mediatedimmunity to Toxoplasma gondii [ J]. J Immunol, 2000, 165 (10):5720 - 5727.
  • 7Ponnappan U, Trebilcock GU, Zheng MZ. Studies into the effect of tyrosine phosphatase inhibitor phenylarsine oxide on NFkappaB activation in Tlymphocytes during aging: evidence for altered IkappaB-alpha phosphorylation and degradation[J]. Exp Gerontol, 19
  • 8宋淑霞,吕占军.扶正、补肾及益气补肾方药对肾虚老龄小鼠免疫功能的影响[J].细胞与分子免疫学杂志,2002,18(4):387-389. 被引量：13

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中国微生态学杂志
2006年 第5期

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