摘要
用分光光度法研究吖啶橙与肝素钠的结合反应，吖啶橙主要以静电作用的方式与肝素钠发生相互作用。在pH2．0的Britton-Robinson（B-R）缓冲溶液中，吖啶橙在478和492nm处有特征吸收峰，当加入肝索钠后，492nm处的吸光度值强度降低且在453nm处出现了一个新的吸收峰，说明两者之间发生了较强的相互作用形成了一种新的复合物，利用摩尔比法求解两者的结合比为1：3。在最佳条件下，吸光度值的降低与肝素钠的浓度在3．0～15．0mg·L^-1范围内呈良好的线性关系，表观摩尔吸光系数ε=1．599×10^6L·mol^1·cm^-1，检测限（3σ）为0．236mg·L^-1。该方法简单、快速，应用于肝素钠注射液效价的测定，结果令人满意。
The interaction of acridine orange （AO） and heparin was studied by spectrophotometry. In the pH 2.0 Britton-Robinson （B-R） buffer solution, AO had a maximum absorption at 478 nm and 492 nrn. After the addition of heparin, the absorbance value at 492 nm decreased greatly and a new absorption peak at 453 nm appeared, indicating a strong interaction taking place in the reaction solution. Under the optimal condition, the decrease in absorbance at 492 nm was linear with the concentration of heparin in the range of 3.0-15.0 mg ·L^-1. The molar absorptivity was calculated to be ε=1. 599×105 L·mol^-1· cm^-1 and the detection limit was 0. 236 mg·L^-1 （3σ）. The method was furthur applied to the detection of heparin sodium injection samples with satisfactory results. The binding number was calculated to be 1 ： 3 with molar ratio method.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第1期116-119,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(20405008
20635020)
青岛市自然科学基金项目(04-2-JZ-114)
青岛科技大学博士后科学基金项目(0022125)资助
关键词
分光光度法
吖啶橙
肝素
相互作用
Spectrophotometry
Acridine orange
Heparin
Interaction
