摘要
背景:当前应用于引导骨再生的屏障膜不具备骨引导性。目的:探讨极化后的复合生物膜对成骨细胞相容性和活性的影响。方法:采用栅控电晕法制备了壳聚糖/羟基磷灰石复合生物驻极体膜,检测驻极体膜上的表面电位衰减及接种成骨细胞后细胞的增殖分化情况。结果与结论:驻极体膜上的表面电位在第1天内急速衰减,但在接下来的9d内衰减速度逐渐减慢。相较于非极化膜,驻极体膜上的细胞边界明显,有较多的伪足,细胞增殖能力更强,碱性磷酸酶的表达也更高(P<0.05),但肌动蛋白直接免疫荧光结果无显著差异。结果显示该驻极体膜是一种新型的拥有骨引导性的屏障膜,可促进成骨细胞的增殖和分化。
BACKGROUND： Currently, the barrier membranes that applied in guided bone regeneration have no osteoinductivity. OBJECTIVE： To explore the effects of composite bioelectret membranes after polarization on compatibility and activity of osteoblasts. METHODS： Chitosan/nano-hydroxyapatite bioelectret composite membranes were prepared by grid-controlled corona charge. The surface potential decay of the membranes and the proliferation and differentiation of osteoblasts seeded on them were evaluated. RESULTS AND CONCLUSION： The surface potential was obviously reduced in 1 day, but the speed of decay slowed gradually during the following 9 days. Compared with the unpolarized group, the osteoblasts on the bioelectret membranes had clearer boundaries and more pseudopodia. Moreover, the proliferation ability and alkaline phosphatase activity of the cells seeded on bioelectret membranes were higher than those of the unpolarized group （P 〈 0.05）. But the direct immunofluorescence showed no significant difference in actin between the two groups. In conclusion, bioelectret mebrane is a new type of barrier membrane with osteoinductivity and can improve the proliferation and differentiation of osteoblasts.
出处
《中国组织工程研究》
CAS
CSCD
2012年第29期5321-5326,共6页
Chinese Journal of Tissue Engineering Research
基金
教育部高等学校博士学科点专项科研基金(200806101111和20090181120049)~~
