摘要
目的建立一种同时测定复方升麻颗粒(升麻、葛根、白芍、荆芥、防风等)中葛根素、芍药苷和异阿魏酸的反相-高效液相色谱法。方法 Kromasil-C18柱(4.6 mm×150 mm,5μm),乙腈-0.1%磷酸溶液(15∶85)为流动相。采用等度洗脱,同时变体积流量和变波长:1～11 min,体积流量为1.0 mL/mi,波长为230 nm;11.01～20.0 min体积流量为1.5 mL/min,波长为317 nm。结果葛根素、芍药苷和异阿魏酸在上述色谱条件下能完全分离。葛根素、芍药苷和异阿魏酸的线性范围分别为10～160μg/mL、20～320μg/mL和5～80μg/mL;平均回收率分别为100.6%,102.4%和96.5%;RSD分别≤1.9%、≤1.2%和≤1.8%。测得3份样品中葛根素为2.79～2.85 mg/g,芍药苷为15.5～15.7 mg/g,异阿魏酸为1.61～1.64 mg/g。结论该方法操作简便,可同时测定复方升麻颗粒中芍药苷、葛根素、异阿魏酸,精密度和回收率高,重复性好,结果符合要求。
AIM To develop a RP-HPLC method for simultaneously determining the contents of isoferulic acid,puerarin and paeoniflorin in Compound Shengma Granule（Cimicifugae Rhizoma,Puerariae lobatae Radix,Paeoniae Radix alba,Schizonepetae Herba,Saposhnikoviae Radix,etc.）.METHODS Three active compounds were chromatographically separated on a Kromasil column（4.6 mm×15 0 mm,5 μm） by using an isocratic mobile phase composed of CH3CN-0.1%H3PO4（15∶85） coupled with changed flow rate and detection wavelength： 1-11 min：1.0 mL/min,230 nm;11.01-20.0 min：1.5 mL/min,317 nm.RESULTS The peaks of puerarin,paeoniflorin and isoferulic acid were well resolved.They had linear ranges of 10-160 μg/mL,20-320 μg/mL and 5-80 μg/mL with average recoveries of 100.6%,102.4% and 96.5% as well as RSD of ≤1.9%,≤1.2% and ≤1.8%,respectively.Three samples were measured,yielding content of 2.79-2.85 mg/g for puerarin,15.5-15.7 mg/g for paeoniflorin and 1.61-1.64 mg/g for isoferulic acid.CONCLUSION This method is easy to operate and can be used to simultaneously determine the content of the above three compounds and is validated to be of high recovery with good reproducibility.The determined results comply with the requirements.
出处
《中成药》
CAS
CSCD
北大核心
2012年第6期1081-1084,共4页
Chinese Traditional Patent Medicine
