摘要
目的：建立利血通闭颗粒中芍药苷含量的HPLC法测定。方法：采用高效液相色谱法，色谱柱：C18柱，流动相：乙膊-0．1％磷酸溶液（18：82），检测波长为230nm，流速：1．0mL·min-1，柱温为30℃。结果：芍药苷的浓度在0．1483～1．978μg范围内线性关系良好（r=0．9999）平均回收率为98.3％，RSD为0．97％（n=6）。结论：本方法可以简便，快速，准确测定利血通闭颗粒中芍药苷的含量，
Objective： To establish a method for the determination of paeoniflorin in Lixiutongbi particles by HPLC. Methods： An HPLC method was used.The C 18 column was used. The mobile phase was acetonitrile-0.1% phosphoric acid （18：82） at the flow rate of 1.0 ml＇min-1. The detection wavelength was 230 nm and the column temperature was 30 ℃. Results： The linear range of paconitlorin was 0.14832 - 1.9776 μ g（r=0.9999 ）.The average recovery was 98.3% and RSD was 0.97%（n=6 ）. Conclusion： This method is simple, rapid and accurate for the determination of paeoniflorin.
