摘要
目的建立RP-HPLC波长切换法同时测定坤泰胶囊中芍药苷、黄芩苷、盐酸小檗碱、汉黄芩苷、黄芩素、汉黄芩素含量的方法。方法采用RP-HPLC波长切换法同时测定坤泰胶囊中6种活性成分的含量,Waters symmetry C18（5μm,4.6 mm×250 mm）,以乙腈（A）-4%冰醋酸（B）为流动相,梯度洗脱：0~8 min,15%~24%A;8~20 min,24%~31%A;20~35 min,31%~52%A;35~40 min,52%~15%A。体积流量为1.0 ml/min,0~10 min,紫外检测波长为230 nm;10~19 min,紫外检测波长为280 nm;19~21 min,紫外检测波长为345 nm;21~40 min,紫外检测波长为280 nm。柱温30℃,进样体积10μl。结果芍药苷、黄芩苷、盐酸小檗碱、汉黄芩苷、黄芩素、汉黄芩素在0.0834~1.668μg（r=0.9993）、0.3513~7.026μg（r=0.9995）、0.2042~4.084μg（r=0.9994）、0.0735~1.47μg（r=0.9994）、0.0237~0.474μg（r=0.9993）和0.0256~0.512μg（r=0.9993）范围内的线性关系良好,平均回收率分别为99.74%、98.97%、100.71%、100.05%、100.39%和99.55%,RSD分别为2.51%、1.76%、1.92%、2.26%、2.57%、2.54%（n=9）。批号140403坤泰胶囊样品中的6种活性成分分别为0.0794、0.3386、0.1952、0.0716、0.0192、0.0246 mg/ml（RSD均〈3.0%）;批号140803坤泰胶囊样品中的6种活性成分分别为0.0812、0.3446、0.1836、0.0642、0.0212、0.0238 mg/ml（RSD均〈3.0%）。结论本方法简便、快速、准确,重复性好,专属性强,可用于坤泰胶囊的质量控制。
Objective To establish a method for simuhaneous determining the content of paeoniflorin,baicalin,berberine hydrochloride,wogonoside,baicalein and wogonin in Kuntai capsule by RP-HPLC wavelength switching method. Meth- ods RP-HPLC wavelength switching method was used to simultaneous determination of 6 components in Kuntai cap- sule.Waters symmetry Cls （5 μm,4.6 mm×250 mm） was used as chromatographic column,and acetonitrile （A）-4% glacial acetic acid （B） was used for moving phase.From 0 to 8 minutes,A （15%-24%）;from 8 to 20 minutes,A （24%- 31%）;from 20 to 35 minutes,A （31%-52%） and from 35 to 40 minutes,A （52%-15%） were used for gradient elution.The volume flow was 1.0 ml/min.From 0 tol0 minntes,230 nm was used as ultraviolet detection wavelength.From 10 to19 minutes,280 nm was used as ultraviolet detection wavelength.From 19 to21 minutes,345 nm was used as ultraviolet de- tection wavelength.From 21 to 40 minutes,280 nm was used as ultraviolet detection wavelength.Column temperature was at 30℃ and injection volume was 10 μl. Results Within the scope of 0.0834-1.668 μg （r=0.9993）,0.3513-7.026 μg （r= 0.9995）,0.2042-4.084 Ixg （r=0.9994）,0.0735-1.47 μg （r=0.9994）,0.0237-0.474 μg （r=0.9993）and 0.0256-0.512 μg （r= 0.9993）,paeoniflorin,baicalin,berberine hydrochloride,wogonoside,baiealein and wogonin had a good linear relation.The average recovery rate was 99.74%,98.97%,100.71%,100.05%,100.39% and 99.55% respectively.The RSD were 2.51%, 1.76%,1.92%,2.26%,2.57% and 2.54% （n=9） respectively.6 active components was 0.0794,0.3386,0.1952,0.0716,0.0192, 0.0246 mg/ml respectively in Kuntai capsule which batch number was 140403,and all of the RSD less than 3.0%.6 ac- tive components was 0.0812,0.3446,0.1836,0.0642,0,0212,0.0238 mg/ml respectively in Kuntai capsule which batch number was 140803,and all of the RSD less than 3.0%. Conclusion This method had advantages of easy,fast,accuracy, good repeatability and strong specificity.It can ~be used for quality control of kuntai capsule.
出处
《中国当代医药》
2015年第26期7-11,共5页
China Modern Medicine
关键词
坤泰胶囊
同时测定
波长切换法
高效液相色谱法
Kuntai capsule
Simultaneous determination
Changing wave
High-performance liquid chromatography
