摘要
应用HCVC (pC)和HCVCE1E2 (pCE1E2 )重组体转染真核细胞并且通过肌注免疫BALB/C小鼠 ,对其体液免疫和细胞免疫进行检测。所用的 pC和 pCE1E2 均可在真核细胞内表达出特异性HCVC蛋白 ;肌注DNA免疫后均可诱导出BALB/C小鼠的体液和细胞免疫反应 ,抗体反应的A值 :pC组为 0 .358± 0 .0 96 ,pCE1E2 组为 0 .4 15± 0 .12 7;CTL活力 pC组为 18.6 5%± 5.72 % ,pCE1E2 组为 2 0 .0 7%± 11.11% ;通过免疫鼠荷瘤检测体内CTL反应 ,可观察到免疫组鼠发瘤时间滞后 ,发瘤部位减少和存活时间延长。在开发和研制HCV疫苗的过程中 ,DNA免疫是在快速构建、评价和筛选免疫原方面的有效办法。
Two recombinant plasmids were constructed. These include the coding regions for the core protein (pC) and for the core, E 1 and E 2 together (pCE 1E 2). These plasmids were transfected into mammalian cells to test their protein expression and injected into the quadriceps muscles of BALB/C mice to measure specific antibodies and cytotoxic T lymphocyte responses. All the recombinant plasmids were shown to express specific antigens transiently and stably in cells and elicited both specific antibody responses and specific cytotoxic T lymphocyte responses. The A value of anticore were 0.358±0.096 (pC) and 0.415±0.127 (pCE 1E 2). The CTL activity of pC was 18.65%±5.71% and 20.07%±11.11% of pCE 1E 2. Genetic immunization can aid the development of hepatitis C virus vaccines by allowing for the rapid construction and evaluation of different expression plasmids as potential immunogens.
出处
《中国病毒学》
CSCD
2000年第1期14-21,共8页
Virologica Sinica
基金
国家和省自然科学基金!396 70 6 6 5
396 46 1
关键词
丙型肝炎病毒
病毒结构区蛋白
DNA免疫
Hepatitis C Virus
Viral protein of structural regions
DNA immunization
