摘要
目的 研究丙型肝炎病毒 (HCV)核心 (C)基因免疫在诱生特异性细胞免疫应答中的作用。方法 将包含HCVC基因片段的重组真核表达质粒pcDNAHCVC ,在证实其可以在小鼠骨髓瘤细胞SP2 / 0 (H 2 d)中表达之后 ,注射BALB/c小鼠股四头肌。ELISA法检测血清中抗体水平 ;3H TdR掺入法测定免疫小鼠脾细胞特异性增殖能力 ;51Cr释放法检测免疫小鼠细胞毒性T淋巴细胞 (CTLs)体外杀伤功能。结果 8只免疫小鼠血清中均出现了HCVC抗体 ,免疫组小鼠脾细胞增殖能力明显高于对照组 (P <0 .0 0 1) ,增殖指数为 6.10 ,CTLs体外特异性杀伤率为 63 .13 % ,也高于对照组 (P <0 0 1)。结论 HCVC基因免疫可能是防治HCV感染的有效方法。
Objective To study the feasibility of DNA immunization with recombinant expression plasmid containing hepatitis C virus (HCV) core gene against HCV infection. Methods The recombinant plasmid containing HCV C gene was constructed and expressed transiently by using lipofectamine in the mouse SP2/0 cells. After appraisal and purification, these plasmid and control DNA were directly injected intramuscularly into BALB/c mice, HCV C specific antibody in the sera was checked with ELISA. Proliferative responses of spleen cells from experimental and control mice were determined by 3H thymidine incorporation and cytotoxicity T lymphocyte (CTL) activity was measured by standard 4 h 51 Cr release assays. Results All immunized mice developed anti HCV core antibodies and this antibody was dose and immune number dependent. Lymphoproliferative responses for HCV core antigen were higher in immunized mice than in control mice ( P <0.05), stimulation indices (SI) were 2.42～ 6.10. CTLs might induce approximately 63.13% lysis of pcDNA HCV C transfected SP2/0 target cells. Conclusion It is suggested that HCV C gene immunization is a promising method of vaccination against HCV infection.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1999年第7期462-465,共4页
Chinese Journal of Internal Medicine
