摘要
目的 研究丙型肝炎病毒(HCV)的致癌机理。方法 以免疫组化ABC法对46 例肝细胞癌(HCC)及其癌旁肝组织的HCV CP10、NS3及NS5 抗原和ras p21、c-m yc、c-erbB-2、突变型p53 及p16 蛋白进行染色,并将HCC分为HCV抗原阳性组和HCV 抗原阴性组,对比分析HCV 感染对上述癌相关基因蛋白产物表达的影响。结果 46 例HCC患者中,1～3 种HCV 抗原阳性20 例(43.5 % ),2～3 种HCV 抗原阳性16 例(34.8 % ),3 种HCV 抗原阳性9 例(19.6 % )。HCV 抗原阳性组p16 蛋白表达缺失率(80 % ,16/20)明显高于HCV 抗原阴性组(42.3 % ,11/26)(P<0.025),而其他癌相关基因产物在HCV 抗原阴阳性组间的表达则无明显差异。结论 重庆地区约三分之一左右肝癌的发生可能与HCV 感染有关;HCV
Objective To study hepatocarcinogenesis of hepatitis C virus (HCV). Methods Expression of HCV antigens(CP10,NS3,NS5) and several cancer associated gene protein products (ras p21,c myc,c erbB 2,mutated p53 and p16) in the tissues of hepatocellular carcinoma (HCC, n =46) and its surroundings was studied with immunohistochemical method avidin biotin complex. The effect of HCV infection on expression of these gene protein products in HCC was analyzed by comparing HCV antigen positive group with HCV antigen negative group. Results Positive staining of one or two or three HCV antigens was found in 20 cases (43.5 %),of two or three HCV antigens in 16 cases(34.8 %), and of 3 HCV antigens in 9 cases (19.6 %). Deletion rate of p16 expression in HCC with HCV antigen positive (80 %, 16/20) was significantly higher than with HCV antigen negative.While no significance of the other gene product expression was observed between the two groups.Conclusion HCV appears to be related to about one third cases of HCC in Chongqing, southwest of China,and it may involve in hepatocarcinogenesis by inhibiting the function of p16 gene,which acts as a negative regulator of cell cycle.$$$$
出处
《肿瘤》
CAS
CSCD
北大核心
2000年第1期40-42,共3页
Tumor
关键词
肝细胞癌
丙型肝炎病毒
癌基因
肿瘤抑制基因
Hepatocellular carcinoma
Hepatitis C virus
Oncogenes
Tumor suppressor gene
Immunohistochemistry
