摘要
目的 探讨胃癌浸润转移的病变机制。方法 采用免疫组化LSAB法检测 2 0例正常胃组织及 96例胃癌标本中ColⅣ、LN、FN的表达。结果 ColⅣ、LN、FN免疫反应物呈棕色均匀分布在胃腺体周围形成线状基底膜样结构。在增生的纤维组织中则呈不规则条纹状表达。 2 0例正常胃组织的腺体周围基底膜结构均匀、完整 ,基本无间质增生。在 96例胃癌组织中 ,腺体周围基底膜结构遭到明显的破坏 ,呈现断线状、条索状、甚至碎片状表达 ,且出现不同程度的间质增生。本研究表明 :临床晚期的 (39 6 % )、分化程度较低的 (36 5 % )、大体浸润型的 (5 3 1% )、以浸润方式生长的 (6 3 5 % )及出现淋巴结转移的 (6 9 8% )胃癌组 ,基底膜明显缺损及间质明显增生程度大于临床中早期的、中高分化的、大体局限型的、以膨胀方式生长的及未发生淋巴结转移的胃癌组 (全部P <0 0 5 )。结论 胃癌恶性程度的高低与胃腺体周围基底膜结构是否完整有必然联系 ,其浸润转移必首先破坏基底膜。细胞外间质纤维组织的增生一方面可为胃癌细胞浸润生长提供依托 ,另一方面也反映了机体对抗和限制肿瘤转移的一种趋势。提示ColⅣ、LN、FN的免疫组化检测可以作为临床判断胃癌恶性程度、淋巴转移趋势及评估其预后的有价值指标。
Objective To study the pathogenesis of infiltration and metastasis of gastric carcinoma. Method The expressions of collagen Ⅳ,laminin and fibronectin in 20 normal gastric tissue and 96 gastric cancer tissue specimens were detected with LSAB method. Results Appearing brown immunoreactants of collagen Ⅳ,laminin and fibronectin were well-distributed around the gastric glands,forming a linear basement membrane structure.In the hyperplastic fibrotic tissue,they presented irregular brown-line.Among the 20 normal gastric tissue specimens,the basement membrane structure surrounding gastric glands was fairly even and integral,and generally there were not hyperplasia of matrix.Whereas among 96 neoplastic tissue specimens,this membranous structure was subjected to obvious destroy,and hyperplasia of matrix enhanced.Meanwhile,the heavily defective basement membrane and apparently hyperplastic matrix in these groups of the clinical late-term(39 6%),poor histologic differentiation(36 5%),infiltrative growth pattern(63 5%), as well as lymph node metastasis(69 8%) were much more frequently observed(all P <0 05). Conclusion The malignant level of gastric cancer is inevitably connected with the integral basement membrane structure around the gastric glands.The infiltration and metastasis of cancer cells firstly have to break this membranous structure,On the one hand,the hyperplastic fibrotic tissue of extracellular matrix can provide a support for the infiltrative growth of cancer cells;on the other hand,this can also reflect a tendency of resisting neoplastic metastasis in human body.Thus,it is infered that the immunohistologic staining of collagen Ⅳ,laminin,and fibronectin can be viewed as a valuable parameter for evaluating malignant level and lymphangial metastatic tendecy of the gastric cancer. [WT5”HZ]
出处
《山西医科大学学报》
CAS
2000年第2期114-118,共5页
Journal of Shanxi Medical University
关键词
胃肿瘤
层粘连蛋白
Ⅳ型胶原
纤维粘连蛋白
stomach neoplasms
collagen
laminin
fibronectins
extracellular matrix
neoplasm metastasis
