摘要
目的研究共同性外斜视内直肌纤维黏连蛋白表达变化，探讨眼外肌中纤维黏连蛋白表达与共同性外斜视发病的关系。方法选择共同性外斜视患者常规手术切除的内直肌段35例为外斜视组，男19例，女16例，年龄2～46岁（平均20．9岁），外斜视度-45°～-90°（平均斜视度-67．5°），有家族史者8例，并将其分成间歇性外斜视和恒定性外斜视2个亚组，分别为16例和19例。正常组选20例角膜移植供体内直肌作为对照，男12例，女8例，年龄15—28岁（平均18．8岁）。用免疫组化sp法标记内直肌纤维黏连蛋白的表达，图像分析系统测定其平均吸光度（A）值。分析纤维黏连蛋白表达含量在正常组、间歇性外斜视和恒定性外斜视两亚组间及不同性别间、阴阳性家族史间的差异，分析其与斜视度、患者年龄之相关性。所得数据采用单因素方差分析、q检验、t检验及直线相关分析进行统计学处理。结果间歇性外斜视亚组的FN平均4值为0．5821±0．0792，恒定性外斜视亚组为0．4729±0．0872，正常组为0．5914±0．0547，其中恒定性外斜组FN表达低于正常组和间歇性外斜组（q=7．0314，6．1177，P〈0．01），正常组与间歇性外斜视组FN表达差异无统计学意义（g=0．5271，P〉0．05）。FN在患者不同性别、有无家族史之间分别比较，差异无统计学意义（t=1．1317，1．6327，P〉0．05），与患者年龄以及斜视度数无关。结论纤维黏连蛋白含量的下降与可能与共同性外斜视发病和发展有关，纤维黏连蛋白可能是内直肌收缩功能的保护因素。
Objective To study the changes of fibronectin in the resected medial rectus muscles in patients with concomitant exotropia and to have a better understanding about the pathomorphological changes of extraocular muscles in the genesis and development of concomitant exotropia. Methods Thirty-five concomitant exotropic patients were chosen as exotropie group and 20 normals were selected as control group. In the exotropic group , the age range 2 to 46 years old （ mean, 20. 9 ys）, deviation range - 45 ° to - 90 °（mean , -67.5°）, 19 males , 16 females, and 8 cases have the family history. Exotropic patients were divided into intermittent exotropie group 16 cases and constant exotropic group 19 cases. In the control group, the age range 15 -28 years old（ mean, 18.8 years） , 12 males and 8 females. All the resected tissues of the medial rectus muscles were obtained from patients with concomitant exotropia during resection surgery and cornea donators. Fibronectin was measured its mean average optical by Sp method of immunohistochemistry using image analyzer. The amounts of fibronectin in the resected muscle of patients were compared with normals, as well as correlated clinical data of patients such as different types of exotropia, age, degree of deviation, gender and positive family history. Results The amounts of fibronectin in the resected medial rectus muscle of patients with constant exotropia （0. 4729 ±0. 0872 ）were significantly lower than those of normals（0. 5914 ＋0. 0547） and intermittent exotropic group （0. 5821 ± 0. 0792） respeetively（q = 7. 0314, 6. 1177, P 〈 0. 01 ）, while patients with intermittent exotropia did not show significantly lower amounts of fibroneetin than those with normals （ q = 0. 5271, P 〉 0. 05 ） . Neither gender nor positive family was correlated with the amounts of fibronectin（t = 1. 1317, 1. 6327, P 〉 0. 05） and the amounts of fihronectin had no correlation with age and degree of deviation. Conclusion The amounts of fibronectin were correlated with the course of development of concomitant exotropia. Some of these changes may play a role in the pathogenesis of exotropia from intermittence to constancy,which indicates a further clue to the nature of this disease.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2012年第9期794-798,共5页
Chinese Journal of Ophthalmology
关键词
外斜视
细胞外基质
纤连蛋白类
动眼肌
Exotropia
Extracellular matrix
Fibronectins
Oculomotor muscles
