摘要
目的探讨胃癌细胞外基质中纤维连接蛋白(fibronectin,FN)的改变及其与胃癌组织微血管生成的相关性。方法应用免疫组织化学EnVisonTM法,对80例胃癌组织和20例正常胃腺体组织中FN的表达、血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达及肿瘤微血管密度(microvessel density,MVD)进行检测。结果FN免疫复合物呈棕黄色分布于胃腺体基底膜及周围的结缔组织间质中。正常组织基底膜中FN染色呈完整线性结构,结缔组织间质中FN染色呈规则的条纹状。胃癌组织基底膜FN的完整线性染色结构被不同程度破坏,结缔组织间质中FN染色加深,形状不规则。胃癌组织中VEGF表达和MVD较正常组织均升高(P<0.01,P<0.05)。胃癌结缔组织间质中FN表达与VEGF表达和MVD均存在正相关性(P<0.01,P<0.05);而胃癌组织基底膜FN线性染色缺损程度与VEGF表达和MVD均无相关性(P>0.05,P>0.05)。结论胃癌结缔组织间质中FN表达的增高可能与其微血管形成和发展过程关系密切,FN与VEGF在胃癌微血管的形成和发展的过程中可能存在相互协同和促进关系。
Objective To investigate the correlation between the expression of fibronectin （FN） in extracellular matrix （ECM） and angiogenesis of gastric carcinoma. Methods The expressions of FN and vascular endothelial growth factor （VEGF） in 20 specimens of normal gastric tissue （normal group） and 80 specimens of gastric carcinoma tissue（gastric carcinoma group） were detected by EnVisonTM immunohistochemical technique. Tumor microvessel density （MVD） was evaluated by using anti-CD34 antibody as an endothelial marker by the same technique as well. Results The immune complex of FN stained in brown were distributed around glands and in connective tissue of gastric specimens. In normal group, the staining of FN formed intact linear structure at basement membrane and presented regular striae form in connective tissue, In gastric carcinoma group, the integrity of linear structure of FN staining at basement membrane were destroyed to different extent and the staining of FN in connective tissue were changed deeper and distributed irregularly. The expression of VEGF and the value of MVD in the gastric carcinoma group was higher than those in normal group＇s（P〈0.01, P〈0.01, respectively）. This study indicated, that in gas- tric carcinoma group, the degree of FN expression in connective tissue had statistically positive correlations with the degree of VEGF expression and MVD value（P〈0.01, P〈0.05, respectively）. Whereas the destruction extent of linear structure of FN staining at basement membrane showed no correlation with VEGF expression and MVD value （P〉0. 05, P〉0. 05, respectively）. Conclusion The higher expression of FN in connective tissue of gastric carcinoma may well play a critical role in its process of angiogenesis and vasculogenesis. There may be an cooperative interactions between FN and VEGF in the process of angiogenesis and vasculogenesis of gastric carcinoma.
出处
《中国普外基础与临床杂志》
CAS
2005年第6期605-608,617,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
胃癌
纤维连接蛋白
血管生成
血管内皮生长因子
微血管密度
Gastric carcinoma Fibronectin Angiogenesis Vascular endothelial growth factor Microvessel density
