摘要
以马铃薯脱毒试管苗茎段为转化受体材料,建立并优化了农杆菌介导的马铃薯遗传转化体系。通过农杆菌介导法将玉米淀粉分支酶基因(Starch branching enzyme b,SBEⅡb)的过表达载体转化马铃薯,接种762个茎段,共获得35株抗性植株。经PCR检测获得了4株转基因阳性植株;对转基因植株进一步进行GUS活性组织化学染色,发现转基因植株的茎段与试管薯均被染上蓝色,表明外源SBEⅡb基因已整合到马铃薯基因组,且正常表达。
The highly effective potato transformation systems were established and optimized using tube potato plantlets as transformation materials in this study.The maize starch branching enzyme gene（SBEⅡb）was transformed into potato using Agrobacterium tumefaciens-mediated transformation method.762 stem sections were infected by Agrobaterium-mediated tumefacien method.4 transgenic plants were obtained by PCR measurement,implying that SBE Ⅱb gene had been integrated into potato genome.It was also found that GUS gene could normally be expressed in stem sections and potato microtubers of the trasgenetic potato plants.
出处
《广西植物》
CAS
CSCD
北大核心
2012年第2期226-230,共5页
Guihaia
基金
国家自然科学基金(31000132)
阜阳师范学院校级自然科学基金(2010FSKJ12)~~
关键词
玉米淀粉分支酶基因
马铃薯
遗传转化
淀粉
maize starch branching enzyme gene
potato
genetic transformation
starch
