摘要
目的:观察赤雹根总皂苷的抗炎活性,为其研究开发提供实验依据。方法:采用二甲苯致小鼠耳肿胀法,角叉菜胶致小鼠足肿胀法,小鼠棉球肉芽肿法,评价赤雹根总皂苷的抗炎作用。二甲苯耳肿胀法和角叉菜胶致足肿胀法,各组小鼠每日灌胃给药1次,连续3 d,末次给药后30 min,复制模型。小鼠棉球肉芽肿实验,棉球植入术后小鼠连续灌胃给药7 d。实验动物均分为赤雹根总皂苷高、中、低(240,120,60 mg·kg-1)剂量组、阿司匹林(100 mg·kg-1)组、模型组(生理盐水)。结果:赤雹根总皂苷高、中、低剂量组小鼠的耳肿胀度[(1.46±0.19),(1.68±0.15),(1.99±0.17)mg]较模型组(2.31±0.24)mg明显降低(P<0.01);赤雹根总皂苷各剂量组小鼠足肿胀度[(28.7±5.03),(33.8±4.65),(43.33±4.98)mg]较模型组(55.18±2.96)mg明显降低(P<0.01);赤雹根总皂苷各组小鼠肉芽肿质量(5.08±0.37,6.76±0.44,8.66±0.55)mg较模型组(12.05±1.22)mg明显降低(P<0.01),且各实验中赤雹根总皂苷高和(或)中剂量效果优于阿司匹林(P<0.05～0.01)。角叉菜胶致小鼠足肿胀实验中,赤雹根总皂苷各组小鼠血清前列腺素E2(PGE2)吸光度值(0.193±0.022,0.219±0.035,0.250±0.037)和一氧化氮(NO)含量(5.85±3.50,9.62±3.61,16.79±3.14)μmol·L-1较模型组PGE2吸光度值(0.545±0.06),NO含量(23.02±3.18)μmol·L-1明显降低(P<0.01)。结论:赤雹根总皂苷有显著的抗炎作用,其抗炎作用与抑制炎症介质PGE2和NO的合成释放有关。
Objective：To evaluate the anti-inflammatory effects of the total saponins from Thladiantha dubia roots（RTDS）.Method： The effects of RTDS on acute and chronic inflammation were observed in the mouse nflammatory models of ear edema induced by dimethy benzene,paw swelling induced by carrageenan and granuloma induced by cotton pellet.Mice were divided into five groups,RTDS-high dose,middle dose and low dose（240,120,60 mg·kg-1）groups,aspirin（asp,100 mg·kg-1） group and the model group（saline）.Mice in ear edema and paw swelling model groups were administrated intragastrically for three days once a day.The models were established in 30 minutes after the last administration.The mice in granuloma model group induced by implantation of cotton pellet were administrated intragastrically for seven days.Result： The degree of ear edema in RTDS groups [（1.46±0.19）,（1.68±0.15）,（1.99±0.17） mg] was lower than that of the model group（2.31±0.24） mg（P0.01）,the degree of paw swealing in RTDS groups [（28.7±5.03）,（33.8±4.65）,（43.33±4.98） mg] was also lower than that of model group（55.18±2.96） mg（P0.01）,the granulomatous weight in RTDS groups [（5.08±0.37）,（6.76±0.44）,（8.66±0.55） mg] was lower than that of model group（12.05±1.22） mg（P0.01）,and the effect of the high and（or medium） dose of RTDS was better than that of aspirin group（P0.05~0.01）.The prostaglandin E2（PGE2） absorbance value（0.193±0.022）,（0.219±0.035）,（0.250±0.037） and nitric oxide（NO） content（5.85±3.50）,（9.62±3.61）,（16.79±3.14） μmol·L-1 of serum in RTDS groups were lower than that of the model group（0.545±0.06）,（23.02±3.18）μmol·L-1）（P0.01）.Conclusion： RTDS has significant anti-inflammatory effects,and this effect may be related to inhibiting synthesis and release of PGE2 and NO.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第8期163-166,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省自然科学基金项目(C2008000655)
河北省高等学校自然科学研究青年基金项目(2010101)
关键词
总皂苷
赤雹根
抗炎
前列腺素E2
一氧化氮
total saponins
the root of Thladiantha dubia
anti-inflammatory
PGE2
NO
