摘要
利用大孔树脂对粘性红圆酵母RM-1产β-胡萝卜素进行分离纯化,得到最佳的吸附和解吸条件。结果表明,最佳吸附树脂为X-5树脂,最佳洗脱剂为乙醚,最佳分离纯化工艺参数为上样质量浓度111.82μg/mL、吸附流速1mL/min、洗脱流速0.5mL/min。经纯化,β-胡萝卜素纯度达到33.29%,与未纯化相比,提高了6.87倍。
The β-carotene produced by Rhodotorula mucilaginosa RM-1 was purified by macroporous adsorption resin and its optimal adsorption and desorption conditions were achieved in this paper.The results showed that X-5 resin was the optimal adsorbent and ether was the optimal eluent.The optimum static adsorption and desoprtion conditions were sample concentration of 111.82μg/mL,flow rate for adsorption of 1 mL/min,and flow rate for desorption of 0.5 mL/min.Under these optimal conditions,the purity of β-carotene was up to33.29% and the purification fold was 6.87.
出处
《食品科学》
CAS
CSCD
北大核心
2012年第6期83-86,共4页
Food Science
关键词
粘性红圆酵母
Β-胡萝卜素
大孔吸附树脂
分离纯化
Rhodotorula mucilaginosa
β-carotene
macroporous resin
isolation and purification
