摘要
采用罗非鱼尾为原料,研究鱼尾脂溶性色素的去除方法及胶原蛋白的提取工艺,并测定胶原蛋白纯度,鉴定胶原蛋白类型。结果表明,去除色素的最佳方案为混合溶剂氯仿-甲醇-水(1:2:0.8,V/V)、料液比1:15(g/mL)、时间36h,此条件下色素去除率达鱼尾湿质量的2.85%;正交试验确定胶原蛋白提取的最佳工艺为盐酸胍浓度3mol/L、胃蛋白酶添加量3.0%(以粗蛋白量计)、时间72h、料液比1:50(g/mL)、提取次数2次,此条件下胶原蛋白提取率达鱼尾湿质量的4.96%,占鱼尾粗蛋白含量的40.39%;提取物中胶原蛋白含量达94.63%,且显示Ⅰ型胶原蛋白的特征。
The remove of lipid-soluble pigments and extraction of collagen from tilapia tails were studied.In addition,collagen purity and type were analyzed.The highest pigment removal rate,2.85%（calculated on wet basis）,was obtained after 36 h of treatment with a mixture of chloroform,methanol and water（1：2：0.8,V/V） at a material-to-liquid ratio of 1：1.5（g/mL）.The optimal conditions for collagen extraction,as determined by orthogonal array optimization,were guanidine hydrochloride concentration 3 mol/L,pepsin dose 3.0%（calculated based on crude protein weight）,material-to-liquid ratio 1：50（g/mL）,and twice repeated extraction for 72 h each time.Under these conditions,the extraction efficiency of collagen was 4.96%（calculated on wet basis）.In tilapia tails,collagen accounted for 40.39% of total crude protein.The extract obtained contained 94.63% of collagen and displayed the characteristics of collagen typeⅠ.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第6期59-64,共6页
Food Science
基金
教育部留学回国人员科研启动基金项目(20091341)
海南省自然科学基金项目(309007)
关键词
胶原蛋白
色素
提取
鉴定
collagen
pigment
extraction
identification
