摘要
采用酶法制备了不同来源的Ⅱ型胶原,并对其理化性质及免疫活性进行了研究。结果表明:3种来源的软骨中均含有丰富的II型胶原,其中鸡胸软骨含量为81.54%,猪膝关节软骨含量为81.31%,羊肋软骨含量为80.94%;氨基酸组成分析和紫外吸收光谱鉴定表明,提取的胶原蛋白为典型II型胶原;SDS-PAGE电泳结果表明,所提取的II型胶原具有较高的纯度;傅立叶红外光谱和园二色光谱分析表明,3种来源的II型胶原在提取的过程中均保持着完整的二级结构;采用MTT法测定的淋巴细胞增殖反应结果表明,3种来源的II型胶原均具有免疫活性,且鸡胸软骨的活性最高,淋巴细胞增值率为52.91%。
Type II collagen was prepared by enzymatic method, and its physical-chemical properties and immu- nological activities were studied. The results showed that the cartilages from three different sources all contained abun- dant type II collagen. Chicken sternal cartilage had 81.54% , pig swine knees cartilage had 81.31% , and sheep car- tilage had 80.94% type II collagen. The amino acid component analysis and UV spectrum testing verified the extrac- ted collagen was type II collagen. SDS-PAGE showed the extracts had a higher purity. Analysis by Infrared Spectrum and circular dichroism （CD） spectrogram revealed that the extracted type II collagen remained its completed seconda- ry structures. Lymphocyte proliferative responses indicated that the extracted type II collagen possessed immunological activities. The immunological activity of collagen of chicken sternal cartilage was the highest, which attained 52.91%.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第2期22-26,共5页
Food and Fermentation Industries
基金
国家高科技研究和发展计划(863计划)(No.2013AA102207)
上海市自然科学基金项目(No.112R1424400)
