摘要
目的:研究应用震动膜过滤技术对葡萄籽提取物中原花青素的分离纯化效果。方法:应用不同膜孔径的薄膜对葡萄籽提取物进行微滤、浓缩等操作,以通量变化为指标考察膜的稳定性,称量法考察滤液的杂质去除情况,并以Folin-Ciocalteau法测定原花青素的含量。结果:震动膜过滤技术可以有效去除葡萄籽提取物中的大分子杂质,提高原花青素的纯度;同时配合浓缩膜,可使分离浓缩连续进行,工序少、效率高、成本低。结论:震动薄膜过滤技术可用于葡萄籽提取物中原花青素的分离、浓缩工艺。
Objective：To investigate the effectiveness of vibratory shear enhanced membrane filtration technology in separating and purifying proanthocyanidin from grape seeds.Method：Membranes with different pore sizes were used to microfiltrate and concentrate grape seed extract.Membrane flux was determined as a measure of membrane stability.The removal of unwanted impurities was evaluated by weighing.Proanthocyanidin content was assayed by Folin-Ciocalteu method.Results：Vibratory shear enhanced membrane filtration effectively removed macromolecular impurities from grape seed extract,consequently increasing the purity of proanthocyanidin,and allowed continuous separation and concentration with few operations,high efficiency and low cost when used in combination with concentration membrane.Conclusions：Vibratory shear enhanced membrane filtration is applicable for the separation and purification of proanthocyanidin from grape seeds.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第6期7-10,共4页
Food Science
基金
中山市科技计划项目(20103A19020103A235)
广东检验检疫局科学计划项目(2012GDK49)
关键词
震动膜过滤
Folin-Ciocalteau法
原花青素
分离纯化
vibratory shear enhanced membrane filtration
Folin-Ciocalteau method
proanthocyanidin
separation and purification
