摘要
对乳酸乳球菌乳酸亚种SQ80随pH的改变吸附乳链菌肽的规律进行了研究,pH6.5时吸附率达91.5%,pH3以下吸附率为0。通过调整pH,使乳酸乳球菌选择性地吸附、释放乳链菌肽,达到了较好的分离纯化效果,HPLC检测显示单一尖峰,乳链菌肽回收率58.2%,纯化倍数75。
The adsorption rate of nisin to Lactococcus lactis subsp. Lactis SQ80 at different pH conditions was studied. AtpH 6.5, it was 91.5%, the biggest one, but at pH<3.0, nisin wasn’t adsorbed at all. By changing pH , SQ80 cells could adsorbor release nisin selectively. The efficiency of isolation and purification was high. HPLC showed the single peak.The spelifil activityof nisin was increased 75 fold with a yield of 58.2%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2003年第9期103-106,共4页
Food Science
关键词
乳酸乳球菌乳酸亚种
乳链菌肽
分离纯化
吸附率
Lactococcus lactis subsp. Lactis
nisin
isolation and purification
adsorption rate
