摘要
通过静态吸附、静态解吸及吸附动力学研究,对比分析了AB-8、D101、NKA-Ⅱ和NKA-9等4种大孔吸附树脂对夏枯草乙醇提取物中总黄酮的分离纯化效果,并且考察和优化了AB-8树脂分离纯化夏枯草中总黄酮的工艺条件。结果表明,弱极性树脂AB-8的吸附率为90.29%,解吸率为81.29%,是性能良好的总黄酮吸附剂,其最佳条件为:上样浓度3.732 mg.mL-1,pH为5左右,上样流速2 mL.min-1,洗脱液乙醇体积分数40%,解吸液体积5 BV,解吸流速1.5 mL.min-1。经AB-8大孔树脂纯化后,夏枯草乙醇提取物中总黄酮的纯度由46.25%提高到81.58%。
The separating and purifying purpose of the flavonoids from Prunella vulgaris L.was studied and compared with four kinds of macroporous resins including D101,AB-8,NKA-Ⅱand NKA-9.The adsorption rate,desorption rate and adsorption kinetics of each resin were studied,and some technical conditions were sptimized via static and dynamic experiments.The results showed that the weak polar AB-8 macroporous resin was good sorbent of the flavonoids,its adsorption rate and desorption rate were 90.29% and 81.29%,respectively.The optimum conditions for separating and purifying the flavonoids by AB-8 resin were：injecting concentration 3.732 mg·mL-1,pH=5,injecting velocity 2.0 mL·min-1,40%（v/v） alcohol as desorption solvent,desorption velocity of flow 1.5 mL·min-1 and elution volume 5 BV.The content of flavonoids in the purified extracts reached 81.58% from about 46.25%% in the crude extracts after AB-8 resin in the optimized conditions.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2010年第5期451-454,共4页
Journal of Nanchang University(Natural Science)
基金
江西省自然科学基金资助项目(2009GZH0069)
南昌大学食品科学与技术国家重点实验室资助项目(SKLF-TS-200917
SKLF-MB-200807)
关键词
夏枯草
大孔吸附树脂
分离纯化
Prunella vulgaris L.
macroporous resin
separation and purification
