摘要
目的 观察胃癌特异性转移因子 (GSTF)对淋巴细胞进行诱导刺激后 ,淋巴细胞表面的 IL- 2受体 (IL- 2 R)表达的变化 .方法 应用流式细胞仪检测异硫氰基荧光素 (FITC)标记的淋巴细胞表面 IL - 2 R抗体的荧光强度 ,以观察其受刺激前后表面 IL- 2 R的表达 .结果 GSTF在与其特异性抗原共同存在下诱导刺激淋巴细胞 ,可使其表面的 IL- 2 R表达量增加 ,且 GSTF的这种作用具有抗原特异性 ,GSTF或胃癌抗原单独存在时此作用则不明显 .结论 为肿瘤特异性转移因子的活性检测建立了一种新型快速、客观科学的检测方法 ,也为今后肿瘤特异性转移因子的分离纯化及进一步研究转移因子的作用机制打下了基础 .
AIM After lymphocytes stimulated by gastric carcinoma specific transfer factor (GSTF) in vitro , the expression of IL 2 receptor on lymphocytes was studied. METHODS The IL 2 receptor on lymphocytes was detected by flow cytometry using IL 2 receptor's specific antibody labeled by FITC. RESULTS When the lymphocytes were stimulated together with GSTF and gastric carcinoma specific antigen, the expression of IL 2 receptor increased from 6.2% to 17.9%. The activity of GSTF was antigen specific. CONCLUSION Antigen specific transfer factor can induce the expression of IL 2 receptors on lymphocytes. A new, rapid and scientific method is established for the detection of transfer factor activity and for further study of its mechanism.
出处
《第四军医大学学报》
2000年第1期69-71,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金!( 3 982 5 113 )
