摘要
目的 研究急性 T淋巴细胞白血病细胞系 JM分泌的抑制因子 (TL SFJM)在体外对 T细胞凋亡及细胞周期的影响 .方法 以 TL SFJM作用于有丝分裂原 PHA、蛋白激酶 C(PKC)活化剂 PMA和同种异体抗原 (alloantigen)活化的人或小鼠 T细胞 ,应用流式细胞仪检测细胞凋亡及细胞周期 .结果 TL SFJM在体外可诱导 PHA/IL - 2活化的小鼠胸腺细胞的凋亡 .TL SFJM在体外可诱导 alloantigen活化的人外周血单个核细胞 (PBMC)的凋亡 ,且把细胞周期阻遏在 G1期 .TL SFJM对 PMA诱导的人 PBMC活化抗原 CD2 5的表达无明显影响 ,也不引起凋亡 .结论 TL SFJM在体外可诱导 PHA、alloantigen活化的 T细胞凋亡 .
AIM To study the in vitro effect of JM acute T leukemia cell line derived suppressor factor (TLSF JM ) on apoptosis and cell cycle of T cells. METHODS PHA, PMA and alloantigen activated human or mouse T cells were treated with TLSF JM and the apoptosis and cell cycle of the treated cells were determined by FCM. RESULTS ① TLSF JM could induce apoptosis of PHA plus IL 2 activated mouse thymocytes; ② TLSF JM could induce apoptosis of alloantigen activated human peripheral blood mononuclear cells (PBMC) and hold the cell cycle at G1 phase; ③ TLSF JM neither influenced the CD25 expression nor induced apoptosis of PMA activated human PBMC. CONCLUSION TLSF JM can induce apoptosis of alloantigen and PHA activated T cells.
出处
《第四军医大学学报》
2000年第3期268-271,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金!(3 9270613)
