摘要
[目的]研究鹅绒委陵菜多糖(PAP)对荷瘤小鼠外周血细胞因子白介素-2(TL-2)和肿瘤坏死因子-α(TNF-α)的影响。[方法]建立肉瘤S180小鼠动物模型,灌胃给予50、100和200 mg/kg剂量的PAP,以抑瘤率为指标,考察鹅绒委陵菜多糖的体内抗肿瘤活性,测定其抑瘤率;ELISA法测定荷瘤小鼠血清中白细胞介素-2(IL-2)和肿瘤坏死因子-α(TN-α)的水平。[结果]低、中、高3个剂量的PAP对S180荷瘤小鼠的抑瘤率分别为46.41%、52.29%和59.48%;其中CY和低剂量的多糖组合抑瘤率与中、高剂量的多糖抑瘤率相当,并明显高于单用环磷酰胺组的抑瘤率;低、中、高3个剂量的PAP能明显提高荷瘤小鼠血清中IL-2、TNF-α的水平。[结论]鹅绒委陵菜多糖具有提高荷瘤小鼠细胞免疫的作用。
[ Objective ] To study the effects of polysaccharides from Potentilla anserine （PAP） on the levels of interleukin-2 （IL-2） and tumor necrosis factor-a （TNF-a） from peripheral blood. [ Method] The mice models with tumor S180 were established and treated with 50, 100 and 200 mg/kg PAP by gastric perfusion, respectively. The inhibition rate of $180 tumor was measured to investigate the antitumor activity in vivo of PAP. The levels of IL-2 and TNF-a in mice peripheral blood were determined with ELISA. [ Result ] The tumor inhibition rates were 46.41%, 52.29% and 59.48% in different groups treated with 50,100 and 200 mg/kg PAP in vivo, respectively. Of these, the inhibition rate of CY and low-dose PAP was equal to that of medium-and high-dose PAP, significantly higher than that of cyclophosphamide. Three dose groups of PAP could significantly increase the content of IL-2 and TNF-a spleen index. [ Conclusion] The compound PAP could improve the antitumor activity and immunological function of S180 tumor-bearing mice,
出处
《安徽农业科学》
CAS
2012年第9期5177-5178,5180,共3页
Journal of Anhui Agricultural Sciences
基金
国家科技支撑项目(2007BAC18B04)
四川省教育厅重点项目(08ZA095)
关键词
鹅绒委陵菜多糖
抗肿瘤
细胞因子
Potentilla anserine
Polysaccharides
Antitumor
Cytokines
