摘要
目的建立一种操作简单、病变典型、稳定性好的兔在体附件扭转模型并探讨保留卵巢术后卵巢的病理变化和iNOS的改变。方法 40只日本大耳白兔采用随机数字法分为附件扭转(adnexal torsion,AT)模型组(n=32)和对照组(n=8)。模型组兔将左侧附件按顺时针方向扭转3周,避开血管,以4/0丝线横穿扭转形成的3个螺旋圈后固定于左侧腹壁,然后再将之分成4组,每组8只,分别于扭转24、48、72、96h后解除扭转后取双侧卵巢;对照组假手术后96h后取双侧卵巢。所有切除之右侧卵巢组织行病理学研究的内对照,左侧卵巢取1/2行病理检测,另1/2行iNOS生化水平检测。结果左侧附件扭转3周固定左侧腹壁24 h后可见卵巢充血、炎细胞浸润、细胞水肿;48h见较多的炎细胞浸润,细胞结构紊乱;72 h见大量炎细胞浸润,结构损坏和局灶性坏死;96 h见弥漫性细胞坏死;卵巢组织病理评分呈现相同的时相性变化。各组iNOS生化检测水平(左侧vs.右侧),AT组[24 h(3.542±0.987)vs.(1.521±0.214)U/mgprot,P<0.01;48 h(4.986±1.321)vs.(1.832±0.321)U/mgprot,P<0.01;72 h(7.991±1.832)vs.(1.124±0.357)U/mgprot,P<0.01;96 h(6.991±1.227)vs.(1.732±0.572)U/mgprot,P<0.01]。且AT组卵巢均有iNOS不同程度表达,72 h组表达达高峰,96 h下降。结论成功地制备兔附件扭转模型,方法简单、病变典型、重复性好,可模拟女性附件扭转的病理生理过程,对进一步研究附件扭转具有重要意义。初步认为附件扭转72h后卵巢发生不可逆改变,是临床处理附件扭转并保留卵巢的时间临界点。
Objective To establish a rabbit model of adnexal torsion, with typical lesions, good stability, and simple operation, and to explore pathological changes and the role of iNOS in the ovary after preserved surgery. Methods Forty Japanese longear white rabbits were randomly divided into adnexal torsion （AT） model groups （n = 32） and control group （n = 8） using a random digit table. In the model groups, the left adnexes of rabbits were twisted clock wise 1080~ degrees. After that, a 4/0 silk thread was put across the 3 spiral rings of the rotated adnex and sutured to fix it on the left abdominal wall. Then the rabbits in model groups were divided into 4 groups （8 rabbits in each group）. Both o varies of each rabbit were removed at 24, 48, 72, 96 h after the adnexal torsion, respectively. In the control group, the o varies of the rabbits were removed at 96 h after the sham operation. All the right ovaries were removed and served as inter nal control. The left ovaries were taken a half for pathological examination, and the rest for biochemical detection of iNOS. Results At 24 h after the generation of adnexal torsion and its fixisation on the left abdominal wall, ovarian congestion, infiltration of inflammatory cells and edema were seen. More prominent infiltration of inflammatory cells and disorders of cell structure were found after 48 h. After 72 h, a lot of infiltration of inflammatory cells and focal necrosis occurred. Diffuse necosis of cells was observed after 96 h. Pathological examination of the ovarian tissue revealed the same phasic chan ges. Biochemical detection of the iNOS levels in each group （left vs. right） were as follows： 24 h： （3.542 ±0. 987） vs.（1.521±0.214）U/mgprot, P 〈0.01; 48 h： （4.986 ± 1.321） vs. （1.832 ±0.321） U/mgprot, P 〈0.01; 72 h. （7. 991±l.832） vs. （1.124±0.357）U/mgprot, P〈0.01; 96 h： （6. 991sl.227） vs. （1.732s0.572）U/mgprot, P 〈 0.01. In the AT groups, the iNOS expression in the ovaries reached a peak at 72 h and deelined at 96 h after the model ing of ovarian torsion. Conehlslons A rabbit adnexal torsion model has been successfully established. It has favorable features such as simple operation, typical lesions and good reproducibility, and this model well simulates the pathophysiologi eal process of adnexal torsion in women. Threrefore, it may have great significance in further study of adnexal torsion. It is preliminarily concluded that the ovarian injuries become irreversible at 72 h after adnexal torsion, and it is the critical time point of clinical treatment of adnexal torsion with preservation of the ovaries.
出处
《中国实验动物学报》
CAS
CSCD
2012年第1期60-64,I0001,共6页
Acta Laboratorium Animalis Scientia Sinica
关键词
兔
模型
卵巢
扭转
Rabbit
Model
Ovary
Torsion
