摘要
目的:建立高效液相色谱法测定黄芪饮片中4个异黄酮成分的含量方法。方法:色谱柱为Agilent Zorbax SB-C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-水梯度洗脱,流速1.0 ml·min^(-1),检测波长为260 nm,柱温30℃,进样量10μl。结果:毛蕊异黄酮-7-葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素分别在4.42～442μg·ml^(-1)、2.06～206μg·ml^(-1)、3.36～336μg·ml^(-1)、4.60～460μg·ml^(-1)浓度范围内与各自峰面积积分值呈良好的线性关系;平均回收率分别为99.4%(RSD=5.34%)、94.8%(RSD=2.39%)、98.9%(RSD=3.69%)、102.3%(RSD=3.01%)(n=9)。结论:本方法简便、准确、重复性好,可用于黄芪饮片的质量控制。
Objective：To establish an HPLC method for the determination of four isoflavonoids in Radix Astragali from 6 different batches.Method：An Agilent Zorbax ODS column was used with gradient elution composed of acetonitrile and water at a flow rate of 1.0 ml·lml^-1.The detection wavelength was set at 260 nm.Result：The linear range was 4.42-442.00μg -ml^-1 for calycosin-7-oglucoside, 2.06-206.00μg·lml^-1 for ononin,3.36-336.00μg·lml^-1 for calycosin and 4.60-460.00μg·lml^-1 for formononetin.The average recovery was 99.4%（RSD=5.34%）,94.8%（RSD = 2.39%）,98.9%（RSD = 3.69%） and 102.3%（RSD = 3.01%）, respectively（n =9）.Conclusion：The method is simple,accurate and reproducible for the quality control of Astragali Radix.
出处
《中国药师》
CAS
2012年第2期170-172,共3页
China Pharmacist
基金
上海市药学会资助课题(编号:2010-YY-02-12)
关键词
黄芪
异黄酮
高效液相
含量测定
Radix Astragali Isoflavonoids HPLC Content determination
