摘要
目的:优选肠康颗粒的醇沉工艺并从药效学角度考察醇沉工艺的合理性。方法:以除杂率和金丝桃苷、盐酸小檗碱、芍药苷、升麻素苷含量的综合评分为指标,通过单因素试验考察醇沉浓度和清膏相对密度对醇沉工艺的影响,采用小鼠肠推进试验比较肠康颗粒水提清膏醇沉前后的药效。结果:最佳醇沉工艺条件为提取液浓缩至相对密度1.13(70℃)的清膏,加95%乙醇醇沉含醇量85%。结论:优选的醇沉工艺达到了较好的去杂效果并充分保留了药效,为水提醇沉工艺的科学性提供实验依据。
Objective： To optimize alcohol precipitation technology of changkang granule, and investigate rationality of alcohol precipitation technology by pharmacodynamics. Method ： With impurity removal rate and contents of multi-component （hyperoside, berberine, paeoniflorin and prim-O-glucosylcimifugin ） as comprehensive evaluation index, single-factor tests were used to optimize alcohol precipitation technology by taking the concentration of alcohol precipitation and relative density of extract as factors, efficacy of water extract before and after alcohol precipitation was compared through intestinal propulsion test of mice. Result： Optimum alcohol precipitation process was as follows： concentrated extracting liquid to relative density of 1.13 （70 ℃ ） , added 95% ethanol until the concentration of ethanol to 85 %. Conclusion： Good impurity cleaning effect was obtained by optimized alcohol precipitation technology and efficacy was fully kept, it provided experimental basis for scientificity of water extraction-alcohol precipitation technology.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第4期16-19,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省科技支撑计划--社会发展项目(BE2012776)
江苏省中医药研究院新药研发项目(Y1206)
关键词
肠康颗粒
醇沉工艺
药效学
小鼠肠推进试验
单因素试验
Changkang granules
alcohol precipitation technology
pharmacodynamics
intestinal propulsion test
single factor test
