摘要
目的了解南京地区婴幼儿杯状病毒腹泻的感染状况、临床表现以及分子流行病学特征。方法采集2010年7月至2011年6月南京医科大学附属南京儿童医院5岁以下腹泻患儿粪便标本及儿童保健中心健康婴幼儿粪便标本各428份。采用反转录-聚合酶链反应（RT—PCR）检测杯状病毒，测序确定其基因型别。结果428份腹泻样本中有63份为杯状病毒阳性，检出率为14．72％。其中诺如病毒GⅡ型58例，未检出诺如病毒GI型，札如病毒5例，以诺如病毒GⅡ-42006b型为主要流行株。428份健康对照组标本杯状病毒检出19例，诺如病毒6例，札如病毒11例，2例为诺如病毒GⅡ型和札如病毒混合感染。结论南京地区婴幼儿中存在不同基因型杯状病毒感染，流行毒株以GⅡ-2006b为主。
Objective To study the infected information, clinical symptom and molecular epidemiological characteristics of HuCV infection among children under 5 years old in Nanjing. Methods In Nanjing Children＇s Hospital of Nanjing Medical University from July 2010 to June 2011, we collected 428 stool specimens from children with diarrhea and 428 asymptomatic controls. Human Calieivirus were tested by using RT-PCR. Then we sequenced the nucleic acid of PCR amplifications and identified the genotype and gene group of prevalent strains. Results 63 （ 14.72% ） out of 428 stool samples were detected as HuCV. 58 were norovirus and 5 were sapovirus, while G Ⅱ -4 2006b was the predominant strain of NoV. In the 428 control samples, 19 samples were positive for calieivirus, there were 8 NoV and 13 SaV （Including 3 co- infection cases）. Conclusion Human ealiciviruses with different genotypes circulated among children in Nanjing,and G Ⅱ. 2006b is the dominant genotype.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2012年第1期14-17,共4页
Chinese Journal of Experimental and Clinical Virology
基金
南京市医学科技发展重点项目（ZKX09008）
江苏省卫生厅医学科技发展基金（H200949）
关键词
嵌杯病毒科
腹泻
流行病学
分子
Calieiviridae
Diarrhea
Epidemiology,molecular
