摘要
目的:了解天津市地区婴幼儿诺如病毒(NV)感染情况并对诺如病毒进行基因分型分析。方法:收集2008年8月—2009年7月天津市儿童医院门诊和住院病毒性腹泻患儿的粪便标本。用逆转录-聚合酶链反应(RT-PCR)法进行检测。测序后使用Bioedit和DNAstar基因分析软件与GenBank中的参考株进行同源性分析并绘制系统进化树。结果:在540例腹泻标本中诺如病毒阳性的标本为174例,诺如病毒的阳性率为32.22%,2008年11月NV阳性率较高,为62.22%。抽取7份阳性标本进行序列分析,结果7份标本均为GⅡ-4型。结论:诺如病毒是天津地区婴幼儿病毒性腹泻的主要病原体之一,GⅡ-4型是主要流行株。
Objective： To understand the charaterization of Norovirus （NV） infection in infants and young children in Tianjin area, and study the genotype and the genetic identity of NV. Methods： The fecal specimens were collected from infants and young children with viral gastroenteritis from Tianjin Children’s Hospital from August 2008 to July 2009.The viral RNA was amplified by a reverse transcription polymerase chain reaction （RT-PCR）. The amplified genes were sequenced and compared with the NV sequences deposited in GeneBank. The sequence homology was evaluated and the phylogenetic tree was generated by DNAstar and Bioedit software. Results： One hundred and seventy-four positive samples of NV were detected in 540 specimens. The positive rate was 32.22%. There was a higher NV positive rate（62.22%） in November 2008. Seven NV positive samples were selected and sequenced, showing that NV strains all belonged to GⅡ-4. Conclusion： Norovirus is one of the important diarrhea pathogens of viral gastroenteritis in infants and young children in Tianjin area and the GⅡ-4 is the major prevalent strain.
出处
《天津医药》
CAS
北大核心
2010年第11期963-966,共4页
Tianjin Medical Journal
关键词
腹泻
婴儿
基因型
逆转录聚合酶链反应
嵌杯病毒科
序列分析
天津
diarrhea infantile genotype reverse transcriptase polymerase chain reaction caliciviridae sequence analysis TIANJIN
