摘要
目的了解2014年重庆地区诺如病毒（norovirus,NV）感染所致儿童急性腹泻的感染现状、流行基因型以及发生基因重组情况。方法 2014年在重庆医科大学附属儿童医院门诊检验科收集符合纳入条件的腹泻标本511例,采用RT-PCR分别对NV GⅠ和GⅡ基因组的衣壳蛋白区进行扩增、测序,并对序列进行基因分型及构建进化树;为进行重组分析,再次对NV阳性标本的RdRp区和capsid区同时进行扩增,并用Sim Plot进行重组鉴定。结果共收集到符合条件的标本511例（男性290例,女性221例）,检出NV GⅡ型67例,NV检出率为13.1%;8~10月为NV检出高峰,阳性率最高的年龄组为7~12月龄。67例NV阳性标本在capsid区测序成功,检测到5种基因型,依次是GⅡ.4 Sydney 2012型42例（62.7%）,GⅡ.3型8例（11.9%）和GⅡ.17型8例（11.9%）,GⅡ.6型7例（10.4%）,GⅡ.14型2例（3.0%）。59例NV病毒阳性标本同时在RdRp区和capsid区测序成功,共检测到52例重组株,4种重组型分别是GⅡ.e/GⅡ.4 Sydney 2012型42例、GⅡ.12/GⅡ.3型6例、GⅡ.7/GⅡ.6型2例、GⅡ.7/GⅡ.14型2例。结论 2014年重庆地区NV检出率为13.1%,GⅡ.4 Sydney 2012为最主要流行基因型,且NV重组现象十分明显。
Objective To investigate the prevalence of norovirus-associated acute viral diarrhea in children from Chongqing in the year of 2014,and analyze the genotypes and recombination. Methods All norovirus-associated diarrhea samples were collected from children with acute viral diarrhea admitted in our hospital during the year of 2014. RT-PCR was used to amplify the capsid region of NV GⅠ and GⅡ. All the positive products were sequenced,and the obtained sequences were analyzed for genotyping and phylogenetic tree. To study the NV recombination,both RdRp and capsid regions of all the positive products were amplified,and the recombination was identified by Sim Plot software. Results There were 511 specimens collected from 290 male and 221 female children,and 67 of them positive to NV GⅡ,with a positive rate of13. 1%（ 67 /511）. The NV epidemic peak was from August to October,and the peak age group was between7 to 12 months old groups. Five genotypes based on capsid gene diversity were identified in 67 norovirus strains,including G Ⅱ. 4 Sydney 2012（ 42 /67,62. 7%）,G Ⅱ. 3（ 8 /67,11. 9%）,G Ⅱ. 17（ 8 /67,11. 9%）,G Ⅱ. 6（ 7 /67,10. 4%）,and G Ⅱ. 14（ 2 /67,3. 0%）. Fifty-nine norovirus strains were sequenced successfully in the both RdRp and Capsid regions at the same time. Fifty-two specimens were recombination strains,and 4 recombination types of NV GⅡ were detected,including GⅡ. e / GⅡ. 4 Sydney2012（ 42 /52,80. 8%）,GⅡ. 12 / GⅡ. 3（ 6 /52,11. 5%）,GⅡ. 7 / GⅡ. 6（ 2 /52,3. 8%）,and GⅡ. 7 /GⅡ. 14（ 2 /52,3. 8%）. Conclusion The positive rate of NV is 13. 1% in Chongqing region in the year of2014,GⅡ. 4 Sydney 2012 is the predominant strain,and NV recombination is very common.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2016年第1期101-106,共6页
Journal of Third Military Medical University
基金
“十二五”国家科技重大专项(2012ZX10004212-003)~~
