摘要
目的:建立有毒中药京大戟与甘遂生品、醋制品中大戟二烯醇的含量测定方法,并比较两者炮制前后的含量变化。方法:采用高效液相色谱法。色谱柱为Hypersil ODS(250mm×4.6mm,5μm),流动相为乙腈-水(98∶2),柱温为30℃,流速为1.0mL·min-1,检测波长为210nm。结果:大戟二烯醇进样量在0.42～6.23μg范围内与峰面积积分值呈良好线性关系(r=0.9998);京大戟和甘遂生品的平均加样回收率分别为99.28%和99.80%,RSD分别为1.69%和1.83%(n=9)。京大戟中大戟二烯醇的含量明显高于甘遂,且醋制不会对大戟二烯醇的含量造成显著影响。结论:本方法能准确测定京大戟和甘遂中大戟二烯醇的含量。
OBJECTIVE： To develop a method for the determination of euphol in unprocessed Euphorbia pekinensis and E. kansui and their vinegar-processed products, and to compare the changes of euphol before and after process. METHODS： HPLC method was adopted. Samples were analyzed on a Hypersil ODS（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-water （98：2） at a flow of 1.0 mL.min-1 and the detection wavelength was 210 nm. The temperature of column was 30 ℃. RESULTS： The linear range of euphol was 0.42-6.23 ug（r=0.999 8） and the average recovery of E. pekinensis and E. kansui was 99.28% and 99.80% with RSD of 1.69% and 1.83% （n=9）, respectively. The content of euphol in E. pekinensis was higher than in E. kansui significantly. Vinegar process had no significant effect on the content of euphol. CONCLUSION： The method was accurate for the determination of euphol in E. pekinensis and E. kansui.
出处
《中国药房》
CAS
CSCD
2012年第11期1031-1033,共3页
China Pharmacy
