摘要
目的:探讨月腺大戟水提物的毒性作用。方法:以实验用一级标准昆明种近交系小鼠为实验对象,随机分为5组。阴性对照组,给予蒸馏水灌胃;阳性对照组,腹腔注射环磷酰胺50 mg.kg-1;低、中、高剂量组,分别给予77、1442、88 g.kg-1月腺大戟水提物灌胃,观察微核率、精子畸变率以及高剂量组的肝、脾、肾、心、脑、肺病理切片。结果:高剂量(288 g.kg-1)月腺大戟水提物对脾脏、肾脏和心脏具有毒性作用;中、高剂量组的微核率和精子畸变率与阴性对照组均有统计学差异(P<0.01,P<0.005),低剂量组与阴性对照组无统计学差异(P>0.05)。结论:月腺大戟水提物在较高剂量下有致突变和生殖毒性,但在低剂量下未发现明显毒性。
Objective： To investigate the potential toxicity of Euphorbia ebiacteolata Hayata （EEH） water extract. Methods： The study was performed on inbred Kunming mice, using the traditional toxicological method based on observing ratios of micronucleus and sperm abnomlity （RMS）. The mice were randomly divided into 5 groups： a negative control with distilled water, a positive control with cytoxan（50 mg·kg^-1 ）, and low dose, medium, and high dose of EEH extract（77 g·kg^-1, 144 g·kg^-1 and 288 g·kg^-1 respectively）. Results： The results showed that the high and medium doses significantly increased RMS compared with the negative controls （ P 〈 0.01, P 〈 0.005）, but a similar result was not observed in the low dose group （ P 〉 0.05）. Conclusion： EEH can induce mutation and sperm abnormity in medium and high doses, whereas it cannot induce significant toxicity in low doses.
出处
《山东大学学报（医学版）》
CAS
北大核心
2007年第1期62-64,67,共4页
Journal of Shandong University:Health Sciences
关键词
月腺大戟
突变
毒性
小鼠
Euphorbia ebiacteolata Hayata
Mutation
Toxicity
Mice
