摘要
二氢黄酮醇还原酶(DFR)是植物花色苷生物合成途径中的关键酶。以红花石榴"泰山红"的花瓣总RNA为模板,利用简并引物,采用RT-PCR方法,扩增出一条长446 bp的cDNA片段,序列分析结果表明,该cDNA片段与GenBank中的甜樱桃、葡萄等物种的DFR氨基酸同源性为78%～81%,说明该二氢黄酮醇还原酶片段为石榴花DFR基因的cDNA片段,GenBank登录号为JN316028。
Dihydroflavonol 4-reductase（DFR）is the key enzyme in the biosynthesis of anthocyanidins.With the total RNA from red-flower pomegranate ＂Taishanhong＂ as template and using degenerate primers,a new cDNA fragment of 446 bp was amplified by RT-PCR.The sequence analysis demonstrated that the deduced amino acid sequence shared 78%～81% homology with that of sweet cherry,grape and etc.These results suggested that the cloned cDNA fragment was from DFR gene of red-flower pomegranate,and its accession number in GenBank was JN316028.
出处
《山东农业科学》
2012年第2期1-4,共4页
Shandong Agricultural Sciences
基金
山东省自然基金项目(ZR2009DM010)
国家外专局项目[鲁外专(2010)18号]
山东省农业科技成果转化项目[鲁科农字(2010)79号]资助
关键词
石榴
DFR基因
克隆
序列分析
Pomegranate
DFR gene
Clone
Sequence analysis
