摘要
本实验分别以ESR自旋标记法、TBA比色法测定大鼠离体心脏缺血,再灌注时线粒体膜流动性及脂质过氧化物含量。发现心肌线粒体膜深层的流动性在缺血40分钟后有氧再灌注20分钟时(S=0.112±0.006、τ_e=7.13±0.09×10^(-10)sec)明显低于富氧灌注组(S=0.103±0.007、τ_e=6.86±0.20×10^(-10)sec),P<0.05;而脂质过氧化物含量再灌注组(3.02±0.68nmol/mgpr.)则极明显高于富氧灌注组(1.94±0.35nmol/mgpr.),P<0.01。预先给予丹参素,对以上变化有明显的改善作用,上述两项指标与富氧灌注组相比,无明显差别,P>0.05。另外,采用ESR自旋捕集技术发现丹参素对外源性O_2^-·有清除作用。因而推测,丹参素可能作为一种O_2^-·的清除剂保护心肌线粒体免受氧自由基引发的脂质过氧化损害。
The membrane fluidity of mitochondria isolated from langendorff perfused rat hearts was studied with ESR spin labeling technique and TBA method was used to detect the content of lipid peroxide. After total ischemia for 40min followed by a 20min reperfusion the 10-DSA region of the mitochondrial membrane showed significantly increased S (0.1(?)2±0.006) and τ_c(7.13±0.09×10^(10)sec) values (i. e. a decrease of membrane fluidity)in comparison with aerobic control (S=0.103±0.007); τ_c=6.86±0.20×10^(-10) sec) (P<0.05). But lipid peroxide content (3.02±0.68nmol/mgpr.) in the mitochondrial fraction during reperfusion was increased significantly in comparison with aerobic control (1.94±0.35nmol/mgpr.) (P<0.01). The membrane fluidity and lipid peroxide content of reperfused hearts were markedly improved when the hearts before ischemia pretreated with DS-182 in comparison with aerobic control (P>0.05). In addition, we found that DS182 could remove O_2~τ produced by O_2~τ generating system. Our results have showed that DS-182 is a potent scavenger of superoxide. The protective effect of DS-182 on mitochondria during ischemia/reperfusion might be due to its antioxidative activity。
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1990年第6期420-423,共4页
Chinese Journal of Pathophysiology
关键词
线粒体
心肌
过氧化脂质
丹参素
Mttochondrla
Heart
Myocardium
Lipid peroxide
