摘要
目的:建立HPLC法同时测定野菊花中绿原酸和蒙花苷的含量。方法:色谱柱:Agilent Eclipse XDB-C_(18)(250 mm×4.6 mm,5μm);流动相:甲醇-乙腈-0.05%磷酸溶液,梯度洗脱;检测波长:326 nm;流速:1.0 ml·min^(-1);进样量:10μl;柱温:35℃。结果:绿原酸和蒙花苷的线性范围分别为3.476×10^(-2)～0.695 2μg(r=1.000 0,n=7)和8.957×10^(-2)～1.792μg(r=1.000 0,n=7);提取回收率分别为98.79%(RSD=1.0%,n=6)和99.39%(RSD=0.5%,n=6)。结论:本法操作简便、快速、结果准确,可用于野菊花的质量控制。
Objective： To establish a RP-HPLC method for the simultaneous determination of chlorogenic acid and buddleoside in Flos Chrysanthemi lndici. Method： An Agilent Eclipse XDB-C18 （250mm×4.6mm,5μm） column was used. The mobile phase was methanol-acetonitrile-0. 05% H3PO4 with gradient elution and the detection wavelength was 326 nm. The flow rate was 1.0 ml.min-I, the injection volume was 10μl and the column temperature was 35℃. Result： The linear response of chlorogenic acid ranged from 3.476 × 10-2-0. 695 2 μg （r = 1. 000 0,n =7） ,and that for buddleoside was 8. 957 × 10-2-1. 792 μg （r = 1. 000 0,n =7）. The average recovery of chlorogenic acid and buddleoside was 98. 79% （RSD = 1.0% ,n =6） and 99. 39% （RSD =0. 5% ,n =6） ,respectively. Conclusion： The method is convenient,rapid and accurate, and can be used for the quality control of Flos Chrysanthemi lndici.
出处
《中国药师》
CAS
2011年第12期1753-1755,共3页
China Pharmacist
关键词
野菊花
含量测定
绿原酸
蒙花苷
高效液相色谱法
Flos chrysanthemi indici
Determination
Chlorogenic acid
Buddleoside
HPLC
