摘要
目的：观察腺苷对脊髓损伤后细胞外液兴奋性氨基酸（ＥＡＡ）的影响，研究腺苷对脊髓损伤后的神经保护机制。方法：腹侧压迫损伤法造成大鼠脊髓Ｔ１３ 中度损伤，用微透析法收集伤前１５ ｍ ｉｎ 和伤后即刻，１５，３０，４５ ｍ ｉｎ 脊髓Ｔ１３ 的细胞外液。治疗组伤后立即灌注非特异性腺苷受体激动剂２－氯腺苷（２－ＣＡＤＯ，０．１ ｍ ｍ ｏｌ／Ｌ）。采用高效液相色谱仪荧光检测法检测透析液中谷氨酸（Ｇｌｕ）和天门冬氨酸（Ａｓｐ）含量。结果：伤段脊髓细胞外液中Ｇｌｕ，Ａｓｐ 含量在伤后１５ ｍ ｉｎ 分别升高为伤前的５倍和７倍，４５ ｍ ｉｎ 后降为正常，而２－ＣＡＤＯ治疗组则可显著抑制Ｇｌｕ，Ａｓｐ 的升高（Ｐ＜ ０．０１）。
Objective: To observe the effect of adenosine on extracellular excitatory amino acid (EAA) after spinal cord injury (SCI) so as to explore its neuroprotective mechanism on SCI. Methods: Moderate spinal cord injury of T 13 in rats were abtained by using ventral compression injury model. The extracellular fluids were collected 15 min before injury and after every 15 min till 60 min after injury by using microdialysis. Glutamate and asparate in the samples were analyzed by an HPLC. In treatment group, 2 chloroadenesine (2 CADO,0.1 mmol/L), one of nonspecific adenosine agonists, was given through the probe immediately after injury. Results: The concentration of glutamate and asparate in extracellular fluid increased by 5 and 7 times respectively during the first 15 min after SCI, and decreased to pre injury level 45 min after SCI. Conclusion: Treatment with 2 CADO can inhibit the increase of glutamate and asparate significantly ( P <0 01). Adenosine plays a neuroprotective role partly through inhibiting excitotoxicity of EAA.
出处
《第二军医大学学报》
CSCD
北大核心
2000年第1期71-73,共3页
Academic Journal of Second Military Medical University
基金
上海市科委带头人基金!资助项目(97XD14015)
