摘要
以HLA DRB基因类扩增为参照 ,通过HLA DR2组特异性扩增确定HLA DR2携带者 ,PCR/SSP作亚型分型 ,并用银染PCR/SSCP分析HLA DR2等位基因第二外显子单链构像 ,调查湖南地区汉族群体SLE(系统性红斑狼疮 )患者 5 8例和健康对照 5 9例HLA DR2等位基因分布。结果示 ,HLA DR2与SLE相关 (RR =2 .71,P <0 .0 1) ,HLA DRB1 15 0 1为疾病相关等位基因 (RR =3 .10 ,Pc <0 .0 5 ) ,该群体中检出的另外两种亚型DRB1 15 0 2 ,160 2在两组间频率分布差异无显著性。此外 ,PCR/SSCP分析表明湖南汉族HLA DR2等位基因在第二外显子内不存在其他序列变异。
Fifty eight systemic lupus erythematosus (SLE) patients and 59 normal controls of the Han nationality in Hunan province were involved in this study to analyze the correlation between HLA DR2 group specific amplification in combination with HLA DRB generic amplification PCR/SSCP technique to detect the sequence variation within exon 2 of HLA DR2 alleles outside the sequence specific primer matching positions. The results were that HLA DR2 was strongly correlated with SLE (RR = 2.71, P<0.01); and HLA DRB1*1501 was the allele correlated with disease (RR = 3.01, Pc<0.05). In addition, PCR/SSCP showed that there was not any novel sequence variation in exon 2 of HLA DR2 alleles in the Han nationality in Hunan province. [
出处
《湖南医科大学学报》
CSCD
2000年第1期15-17,共3页
Bulletin of Hunan Medical University
关键词
HLA-DR2
SLE
红斑狼疮
系统性
基因多态性
HLA DR2 antigen
lupus erythematosus
systemic
polymerase chain reaction
single strand comformational polymorphism
