摘要
目的:观察离心训练和向心训练对大鼠腓肠肌乙酰胆碱(ACh)、烟碱型乙酰胆碱受体(nAChR)含量的影响,探讨不同训练方式对大鼠神经肌肉适应性的影响。方法:雄性SD大鼠30只,随机分为离心训练组、向心训练组和对照组。离心训练组采用-14°的下坡跑训练,每天进行1次速度为17～21 m/min、总时间25～40 min的训练;向心训练组采用4°上坡跑训练,每天进行1次速度为19～23 m/min、总时间为25～40 min的训练。两组大鼠均持续训练8周。对照组不参加训练,正常饮食。在训练组最后一次运动结束24小时,麻醉3组大鼠并取材,采用Elisa技术检测大鼠左侧下肢腓肠肌ACh、nAChR含量。结果:离心训练组和向心训练组ACh含量均显著高于对照组。离心组nAChR含量显著高于对照组。结论:离心训练和向心训练对大鼠神经肌肉适应性产生不同影响,离心训练影响更显著。
Objective The purpose of this study is to investigate the neuromuscular adaptation of rats to eccentric training and concentric training through the changes in the content ofACh （acetylcho- line）. , and nAChR （nicotinic acetylcholine receptors） . Methods Thirty male SD rats were randomly divided into the eccentric training group （EE group） , concentric training group （CE group） and con-trol group. -14° downhill running was applied for EE group （25-40 minute at a speed of 17-21 rn/min, once a day） . 4°uphill running was applied for CE group （25-40 minute at the speed of 19-23 m/ min, once a day） . They were continuously trained for 8 weeks. Within 24 hours of ending the training protocol, rats in three groups were simultaneously harvested under anesthesia. The gastrocnemius muscleof the rats was removed and ACh and nAChR in the muscle were tested by Ellsa tectmlques. Results The content of Ach in both exercise groups was significantly higher than that in the control group, and the con- tent of nAChR in EE group was significantly higher than that in control group. Conclusion Both eccentric and concentric training affect the neuromuscular adaptation of rats, especially eccentric training.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2012年第1期38-41,共4页
Chinese Journal of Sports Medicine
